An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections) Competitor A: Leading mouse monoclonalCompetitor B: Non-Abcam rabbit monoclonal ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.
![IHC image of NeuN (ab177487) with Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human cerebellum tissue section.The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (ab177487, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 1.0µg/ml) was then appli](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29184_ab177487-232795-ab177487-01ug-P8D84xC-2M-1ug-x10.jpg)
IHC image of NeuN (ab177487) with Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human cerebellum tissue section.The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (ab177487, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 1.0µg/ml) was then appli
IHC-P image of NeuN (green) and GFAP (red) double staining on mouse cerebellum sections using ab177487 (1/5000) and ab4674 (1/1500) respectively. The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were then incubated with Rabbit Monoclonal to NeuN (ab177487) diluted at 1/5000 and Chicken Polyclonal to GFAP (ab4674) diluted at 1/1500. The primary antibody was detected using ab150097 Goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (1/500) and ab150176 Goat anti-chicken IgY conjugated to Alexa Fluor® 594 (1/500)
IHC-FoFr staining of NeuN staining on mouse pons sections using ab177487 (1/6000). The mouse was perfusion fixed using formaldehyde and 20µm sections were permeabilized with 0.5% tween. Blocking was performed using 1% BSA. ab177487 was diluted 1/6000 and incubated with the sections for 16 hours at 21°C. secondary antibody used was goat polyclonal to rabbit IgG conjugated to Alexa Fluor® 594 (1/1000).See Abreview
IHC-Fr staining of NeuN on zebrafish brain at 4dpf sections using ab177487 (1/100). The sections were fixed in paraformaldehyde and permeabilized using triton X. Antigen retrieval uisng sodium citrate was used. The sections were blocked using 5% BSA for 1 hour at 23°C. ab177487 was diluted 1/100 and incubated for 16 hours at 4°C. The secondary antibody used was anti rabbit IgG conjugated to Alexa Fluor® 488 (1/1000). Dapi used as counterstain.See Abreview
An independent comparison of commercially available NeuN clones in IHC-PCompetitor A: Leading mouse monoclonalCompetitor B: Non-Abcam rabbit monoclonal Sodium citrate was used for antigen retrieval in all 3 samples. ab177487 produces specific staining, equivalent to the leading mouse monoclonal at half the dilution. The non-Abcam mouse monoclonal was less specific as it stained Purkinje cells, which do not express NeuN.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling NeuN with unpurified ab177487 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling NeuN with purified ab177487 at 1/3000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
IHC-P image of FOX3/NeuN staining on dog cerebellum sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
IHC-P image of FOX3/NeuN staining on rat brain (SVZ) sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
IHC-P image of FOX3/NeuN staining on mouse brain (frontal cortex) sections using ab177487 (1/800). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/800 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
IHC-P image of FOX3/NeuN staining on zebrafish spinal cord sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
IHC-P image of FOX3/NeuN staining on marmoset cerebellum sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
IHC-P image of FOX3/NeuN staining on goat cerebellum sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
IHC-P image of FOX3/NeuN staining on cat cerebellum sections using ab177487 (1/1000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
IHC-P image of FOX3/NeuN staining on sheep brain (Frontal cortex) sections using ab177487 (1/1000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10mins at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1/250).
ab177487 staining NeuN in mouse free floating 50 micron lumbar spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 10% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS + Triton) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/700) was used as the secondary antibody.See Abreview
ab177487 staining NeuN in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with Triton X-100 + 0.4% horse seurm for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
ab177487 staining NeuN in Mouse embryonic day 15 brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.5% Triton X-100 in PBS, blocked with 10% serum for 1 hour at 25°C and antigen retrieval was by heat mediation in citrate buffer, pH 6. The sample was incubated with primary antibody (1/500 in PBS + 1% serum + 0.1% Triton X-100) for 16 hours at 25°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit IgG (H+L) polyclonal (1/700) was used as the secondary antibody.See Abreview
![All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/1500 dilution (unpurified)Lane 1 : Human fetal brain tissue lysateLane 2 : Mouse brain tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29202_ab177487-239324-ab177487upwb.jpg)
All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/1500 dilution (unpurified)Lane 1 : Human fetal brain tissue lysateLane 2 : Mouse brain tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
![All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/10000 dilution (purified)Lane 1 : Human fetal brain tissue lysateLane 2 : Mouse brain tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29203_ab177487-239325-ab177487pwb.jpg)
All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/10000 dilution (purified)Lane 1 : Human fetal brain tissue lysateLane 2 : Mouse brain tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
![All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/1000 dilution (unpurified)Lane 1 : Fetal brain lysateLane 2 : 293T lysateLane 3 : HeLa lysateLane 4 : SH-SY5Y lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29204_ab177487-205419-ab177487.jpg)
All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/1000 dilution (unpurified)Lane 1 : Fetal brain lysateLane 2 : 293T lysateLane 3 : HeLa lysateLane 4 : SH-SY5Y lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
![All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1 µg/ml (unpurified)Lane 1 : Brain (Mouse) Tissue LysateLane 2 : Cerebellum Mouse Tissue LysateLane 3 : Cerebellum Rat Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29205_ab177487-224309-FWBab177487GR1418824.jpg)
All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1 µg/ml (unpurified)Lane 1 : Brain (Mouse) Tissue LysateLane 2 : Cerebellum Mouse Tissue LysateLane 3 : Cerebellum Rat Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Immunocytochemsitry/Immunofluorescence analysis of SH-SY5Y cells labelling NeuN (green) with unpurified ab177487 at 1/80. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunocytochemsitry/Immunofluorescence analysis of SH-SY5Y cells labelling NeuN (green) with purified ab177487 at 1/300. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).