RBFOX3

Gene Symbol RBFOX3
Entrez Gene 146713
Alt Symbol FOX-3, FOX3, HRNBP3, NEUN
Species Human
Gene Type protein-coding
Description RNA binding protein, fox-1 homolog (C. elegans) 3
Other Description RNA binding protein fox-1 homolog 3|fox-1 homolog C|hexaribonucleotide binding protein 3|neuronal nuclei
Swissprots B4DF29 B4DEG6 A6NFN3
Accessions A6NFN3 EAW89563 EAW89564 AK054893 AK124644 AK128131 AK293617 BAG57077 AK293905 BAG57290 BC093713 AAH93713 BM714144 BU741507 BX452143 DN990270 XM_011524359 XP_011522661 XM_011524360 XP_011522662 XM_011524361 XP_011522663 XM_011524362 XP_011522664 XM_011524363 XP_011522665 XM_011524364 XP_011522666 XM_011524365 XP_011522667 XM_011524366 XP_011522668 XM_011524367 XP_011522669 XM_011524368 XP_011522670 NM_001025448 NM_001082575 NP_001076044
Function RNA-binding protein that regulates alternative splicing events. {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.

NeuN (D3S3I) Rabbit mAb - 12943 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3S3I
Western blot analysis of extracts from mouse brain, C2C12 cells, and rat brain using NeuN (D3S3I) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Fox-3 (N-14) - sc-246957 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Fox-3 (N-14): sc-246957. Western blot analysis of Hrnbp3 expression in non-transfected: sc-117752 (A) and mouse Hrnbp3 transfected: sc-125473 (B) 293T whole cell lysates.

Fox-3 (S-20) - sc-246958 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-NeuN antibody [EPR12763] - Neuronal Marker - ab177487 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12763
​An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections) Competitor A: Leading mouse monoclonalCompetitor B: Non-Abcam rabbit monoclonal  ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.

Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 488) - ab190195 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12763
Overlay histogram showing U-87MG cells stained with ab190195 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab190195, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in U-87MG fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 647) - ab190565 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12763
ab190565 staining NeuN in NGF-differentiated PC12 cells (7 days). The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190565 at 1/50 dilution (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. 

Anti-NeuN antibody - Neuronal Marker - ab104225 from Abcam

Prices $401.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab104225 staining NeuN in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton-X and blocked with 5% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Anti-NeuN antibody - Neuronal Marker - ab131624 from Abcam

Prices $376.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
Immunofluorescence analysis of cochlear ganglion of a neonatal Mouse labelling FOX3/NeuN with ab131624 at 1/1000 in red. Beta-tubulin in labelled in green.

Anti-NeuN antibody - Neuronal Marker - ab134014 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
IHC-P image of a transgenic mouse expressing the eGFP gene product under control of the Thy 1 promoter. The sections of mouse brain show NeuN (Red) staining using ab134014 (1:1000). The secondary antibody used was anti-chicken conjugated to Cy3 Dye. Green is eGFP autofluorescence.

Anti-NeuN Antibody - LS-C149132 from LifeSpan Bioscience

Prices $475.00
Sizes 1 ml
Host Chicken
Clonality Polyclonal

Anti-NeuN Antibody (clone A60) - LS-C73393 from LifeSpan Bioscience

Prices $560.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone A60

Anti-NeuN Antibody (clone A60) - LS-C312122 from LifeSpan Bioscience

Prices $255.00, $385.00, $515.00
Sizes 100 µl, 500 µl, 1 ml
Host Mouse
Clonality Monoclonal
Clone A60
IHC of NeuN on FFPE Brain tissue.

Anti-NeuN Antibody (clone A60, Ready-to-Use) - LS-C348669 from LifeSpan Bioscience

Prices $255.00, $325.00, $465.00
Sizes 3 ml, 7 ml, 15 ml
Host Mouse
Clonality Monoclonal
Clone A60
IHC of NeuN on FFPE Brain tissue.

Anti-RBFOX3 / NEUN Antibody (Biotin) - LS-C180800 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RBFOX3 / NEUN Antibody (DY650) - LS-C180801 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RBFOX3 / NEUN Antibody (DY488) - LS-C180802 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RBFOX3 / NEUN Antibody (DY550) - LS-C180803 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RBFOX3 / NEUN Antibody IHC-plus™ - LS-B10249 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RBFOX3 / NEUN antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10249 concentration 10 ug/ml.

Anti-RBFOX3 / NEUN Antibody - LS-C192977 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of mouse brain cell lines, using NeuN antibody.

Anti-RBFOX3 / NEUN Antibody - LS-C335111 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded mouse kidney using NeuN antibody at dilution of 1:200 (x400 lens)

Anti-RBFOX3 / NEUN Antibody - LS-C204519 from LifeSpan Bioscience

Prices $285.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde fixed frozen section of adult rat cortex stained with RBFOX3 / NEUN antibody in red and counterstained for DNA in blue. Note that RBFOX3 / NEUN antibody stains neuronal nuclei and distal perikarya, exactly like the original NeuN monoclonal antibody and like NeuN/Fox3 monoclonal IB7. These antibodies do not bind to the nuclei of perikarya of non-neuronal cells, so that they can be used to identify neurons.

Anti-RBFOX3 / NEUN Antibody (clone 1B7) - LS-C204518 from LifeSpan Bioscience

Prices $285.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B7
Rat brain neural cultures stained with RBFOX3 / NEUN antibody (red), chicken polyclonal antibody to GFAP (green) and DNA (blue). The RBFOX3 / NEUN antibody antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells. Note that we have used the same immunogen to generate a rabbit polyclonal antibody to Fox3/NeuN, RPCA-Fox3.

RBFOX3/NeuN Antibody - NBP1-77686 from Novus Biologicals

Prices $99.00, $329.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - NeuN antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI(blue) and Dylight 550 (red).

RBFOX3/NeuN Antibody (1B7) - NBP1-92693 from Novus Biologicals

Prices $99.00, $299.00
Sizes 25 µl, 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B7

RBFOX3/NeuN Antibody - NBP1-92716 from Novus Biologicals

Prices $99.00, $299.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RBFOX/Neun Antibody [NBP1-92716] - Western blot of rat brain homogenates stained with NBP1-92716. Antibody binds closely spaced 48 and 46kDa bands.
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