Anti-NeuN antibody - Neuronal Marker
| Name | Anti-NeuN antibody - Neuronal Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab104225 |
| Prices | $401.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IHC-F IHC-P ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human, Chimpanzee, Monkey, Orangutan |
| Antigen | Recombinant fragment corresponding to Human NeuN aa 1-100 (N terminal) |
| Description | Rabbit Polyclonal |
| Gene | RBFOX3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab104225 staining NeuN in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton-X and blocked with 5% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
IHC-P image of FOX3/NeuN staining using rat DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200).See Abreview
ab104225 at 1/1000 dilution, staining FOX3 (red) in rat brain cortex and counterstained for DNA in blue.
IHC-P image of FOX3/NeuN staining using mouse DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200).See Abreview
ab104225 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104225 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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