β-Catenin Antibody (Carboxy-terminal Antigen)

• Supplier: Cell Signaling Technology
• Catalog: 9587
• Prices: $99.00, $246.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP IHC-P IHC-F ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Chicken, Xenopus, Bovine, Dog, Pig, Horse
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human β-catenin
• Description: Rabbit Polyclonal
• Gene: CTNNB1
• Conjugate: Carboxy-terminal Antigen

Product images

Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin Antibody (Carboxy-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using β-Catenin Antibody (Carboxy-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen) in the presence of control peptide (left) or beta-Catenin blocking peptide #1002 (right).

Immunohistochemical analysis of frozen HCC827 xenograft using beta-Catenin Antibody (Carboxy-terminal Antigen).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT116 cells and either 20 μl of β-Catenin Antibody (Carboxy-terminal Antigen) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP ® Human Axin2 Intron 1 Primers #8973, SimpleChIP ® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.