All lanes : Anti-NeuroD2 antibody (ab104430) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Cerebellum Mouse Tissue Lysate Lane 3 : Mouse Hippocampus Tissue Lysate Lane 4 : P0 Mouse Brain Mouse Tissue LysateLane 5 : P7 Mouse Brain Tissue Lysate Lane 6 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with Immunising peptide at 1 µg/mlLane 7 : Cerebellum Mouse Tissue Lysate with Immunising peptide at 1 µg/mlLane 8 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1 µg/mlLane 9 : P0 Mouse Brain Mouse Tissue Lysate with Immunising peptide at 1 µg/mlLane 10 : P7 Mouse Brain Tissue Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC-P image of NeuroD2 staining on mouse CA1 hippocampus sections using ab104430 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours.See Abreview
IHC-P image of NeuroD2 staining on rat CA1 hippocampus sections using ab104430 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hoursSee Abreview
ab104430 staining NeuroD2 in 10% formaldehyde perfusion fixed 6 week old frozen mouse brain section (dentate gyrus). No antigen retrieval was performed. The section was incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104430, 1/2000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue).