Anti-NeuroD2 antibody [EPR5135] - ChIP Grade

• Supplier: Abcam
• Catalog: ab109406
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5135
• Applications: ChIP WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in Human NeuroD2
• Description: Rabbit Monoclonal
• Gene: NEUROD2
• Conjugate: Unconjugated

Product images

All lanes : Anti-NeuroD2 antibody [EPR5135] - ChIP Grade (ab109406) at 1/1000 dilutionLane 1 : Human cerebellum lysateLane 2 : Human hippocampus lysateLane 3 : Human fetal brain lysatesLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/250 ab109406

ChIP analysis using ab109406 binding NeuroD2 in cerebral cortex tissue lysate from postnatal day 0 mouse. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/200) for 2 hours at 4°C.Positive control: Known target of NeuroD2, which is the promoter regions of Nhlh2 gene.Primer sequence for PCR was:Nhlh2_F CTCACGAACTTCACCCGCACNhlh2_R AAATTGACTCCTCGGCCCTCNegative Control: ChIP performed with unrelated antibody (anti-GFP antibody).See Abreview

Product References

Contribution of microRNA-1275 to Claudin11 protein suppression via a - Contribution of microRNA-1275 to Claudin11 protein suppression via a

Katsushima K, Shinjo K, Natsume A, Ohka F, Fujii M, Osada H, Sekido Y, Kondo Y. J Biol Chem. 2012 Aug 10;287(33):27396-406.