Anti-Neuroglobin antibody [13C8]
| Name | Anti-Neuroglobin antibody [13C8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab37258 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 13C8 |
| Applications | WB ELISA FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | NGB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing SH-SY5Y cells stained with ab37258 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab37258, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29408_Neuroglobin-Primary-antibodies-ab37258-2.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab37258 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab37258, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Anti-Neuroglobin antibody [13C8] (ab37258) at 1 µg/ml + Human Neuroglobin full length protein (ab63278) at 0.1 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29409_Neuroglobin-Primary-antibodies-ab37258-4.jpg)
Anti-Neuroglobin antibody [13C8] (ab37258) at 1 µg/ml + Human Neuroglobin full length protein (ab63278) at 0.1 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes
Product References
Combined antiapoptotic and antioxidant approach to acute neuroprotection for - Combined antiapoptotic and antioxidant approach to acute neuroprotection for
Ord EN, Shirley R, McClure JD, McCabe C, Kremer EJ, Macrae IM, Work LM. J Cereb Blood Flow Metab. 2013 Aug;33(8):1215-24.
Neuroglobin protects nerve cells from apoptosis by inhibiting the intrinsic - Neuroglobin protects nerve cells from apoptosis by inhibiting the intrinsic
Raychaudhuri S, Skommer J, Henty K, Birch N, Brittain T. Apoptosis. 2010 Apr;15(4):401-11.
TAT-mediated delivery of neuroglobin protects against focal cerebral ischemia in - TAT-mediated delivery of neuroglobin protects against focal cerebral ischemia in
Cai B, Lin Y, Xue XH, Fang L, Wang N, Wu ZY. Exp Neurol. 2011 Jan;227(1):224-31.
17beta-estradiol--a new modulator of neuroglobin levels in neurons: role in - 17beta-estradiol--a new modulator of neuroglobin levels in neurons: role in
De Marinis E, Ascenzi P, Pellegrini M, Galluzzo P, Bulzomi P, Arevalo MA, Garcia-Segura LM, Marino M. Neurosignals. 2010;18(4):223-35.
Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons. - Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons.
Ostojic J, Sakaguchi DS, de Lathouder Y, Hargrove MS, Trent JT 3rd, Kwon YH, Kardon RH, Kuehn MH, Betts DM, Grozdanic S. Invest Ophthalmol Vis Sci. 2006 Mar;47(3):1016-23.