Western blot analysis of Neurokinin 1 Receptor in K562 cell lysate immunoprecipitated using ab183713 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of K562 lysates.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
All lanes : Anti-Neurokinin 1 Receptor antibody [EPR6836(2)] (ab183713) at 1/50000 dilutionLane 1 : K562 cell lysateLane 2 : Jurkat cell lysateLane 3 : THP-1 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution