Anti-Neuropilin 1 antibody

• Supplier: Abcam
• Catalog: ab25998
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P ELISA WB
• Species Reactivities: Mouse, Human
• Antigen: ab25998 is specific for Neuropilin 1
• Blocking Peptide: Human Neuropilin 1 peptide
• Description: Rabbit Polyclonal
• Gene: NRP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Neuropilin 1 antibody (ab25998) at 1/500 dilutionLane 1 : Untransfected COS-7 cellsLane 2 : COS-7 cells transfected with HA-tagged Neuropilin 1

ICC/IF image of ab25998 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25998, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab25998 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25998, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Hormonal regulation and distinct functions of semaphorin-3B and semaphorin-3F in - Hormonal regulation and distinct functions of semaphorin-3B and semaphorin-3F in

Joseph D, Ho SM, Syed V. Mol Cancer Ther. 2010 Feb;9(2):499-509.

HIV-1 upregulates VEGF in podocytes. - HIV-1 upregulates VEGF in podocytes.

Korgaonkar SN, Feng X, Ross MD, Lu TC, D'Agati V, Iyengar R, Klotman PE, He JC. J Am Soc Nephrol. 2008 May;19(5):877-83.

Advanced glycation end-products suppress neuropilin-1 expression in podocytes. - Advanced glycation end-products suppress neuropilin-1 expression in podocytes.

Bondeva T, Ruster C, Franke S, Hammerschmid E, Klagsbrun M, Cohen CD, Wolf G. Kidney Int. 2009 Mar;75(6):605-16.