Anti-Neuropilin 1 antibody [EPR3113]

• Supplier: Abcam
• Catalog: ab81321
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3113
• Applications: WB IHC-P IP FC IHC-F ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Monkey, Marmoset
• Antigen: Synthetic peptide (Human) from the cytoplasmic region
• Description: Rabbit Monoclonal
• Gene: NRP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/1000 dilutionLane 1 : Human placenta lysateLane 2 : HUVEC cell lysateLane 3 : HepG2 cell lysateLane 4 : mouse heart tissue lysateLane 5 : mouse kidney tissue lysateLane 6 : rat heart tissue lysateLane 7 : rat kidney tissue lysateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab81321 at 1/100 dilution.

ab81321 staining Neuropilin 1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview

ab81321 staining Neuropilin 1 in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 1 hour at 27°C followed by incubation with the primary antibody, undiluted, for 14 hours at 4°C. An undiluted Cy3®conjugated donkey anti-rabbit was used as the secondary antibody.See Abreview

Overlay histogram showing HepG2 cells stained with ab81321 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81321, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

ICC/IF image of ab81321 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81321, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab81321 staining Neuropilin 1 in the COS1 fibroblast-like cell line derived from monkey kidney tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit monoclonal(1/500) was used as the secondary antibody.See Abreview

Product References

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