Anti-Neutrophil Elastase antibody
| Name | Anti-Neutrophil Elastase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68672 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F IHC-P |
| Species Reactivities | Mouse, Human, Horse, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Neutrophil Elastase |
| Description | Rabbit Polyclonal |
| Gene | ELANE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Neutrophil Elastase staining in human spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68672, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Lanes 1 - 2 : Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/mlLanes 3 - 4 : Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml (Milk blocking has been used)Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 2 : Mouse Bone Marrow Tissue LysateLane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 4 : Mouse Bone Marrow Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunohistochemistical detection of Neutrophil Elastase using antibody (ab68672) on formaldehyde-fixed paraffin-embedded human spleen sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Permeabilization: No Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody dilution 1/2000; incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG conjugated to biotin (1/200).See Abreview
Immunofluorescent staining with ab68672 of blood film of trangenic mouse that suffered from systemic inflamation. Neutrophil Elastase (in green) expressed in the lysosomes of activated neutrophils. Cells were fixed with methanol and permeabilized with 0.3% TritonX in 0.1% PBS. Blocking was performed with 10% serum for 15 minutes at 24ºC. This was followed by 1 hour incubation at 24ºC with ab68672 which was used at 1/200. The secondary antibody used was an anti-rabbit Alexa Fluor 488.See Abreview
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
Product References
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