Anti-Nexilin antibody
| Name | Anti-Nexilin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83746 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Guinea Pig, Bovine, Dog |
| Antigen | Synthetic peptide within internal sequence amino acids 252-301: EELERQRQEN RKKQAEEEAR KRLEEEKRAF EEARRQMVNE DEENQDTAKI, of Human Nexilin (NP_653174) Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | NEXN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Nexilin antibody (ab83746) at 1 µg/ml (in 5% skim milk/ PBS) + Fetal liver lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab83746 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83746, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbir IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.