Anti-NF2 / Merlin antibody
| Name | Anti-NF2 / Merlin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab30329 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB DB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length protein (human) |
| Description | Rabbit Polyclonal |
| Gene | NF2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-NF2 / Merlin antibody (ab30329) at 1/1000 dilutionLane 1 : 3T3 cell lysateLane 2 : Cos7 cell lysateLane 3 : recombinant merlin proteinLane 4 : Normal human Schwann cell (NHSC)lysate
ICC/IF image of ab30329 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30329, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.