NF2

Gene Symbol NF2
Entrez Gene 4771
Alt Symbol ACN, BANF, SCH
Species Human
Gene Type protein-coding
Description neurofibromin 2 (merlin)
Other Description merlin|moesin-ezrin-radixin like|moesin-ezrin-radixin-like protein|moesin-ezrin-radizin-like protein|neurofibromin 2 (bilateral acoustic neuroma)|neurofibromin-2|schwannomerlin|schwannomin
Swissprots Q969N0 Q9UNH3 Q9BTW3 Q96T30 O95683 Q96T33 Q9UNH4 Q96T31 Q9UNG9 Q96T32 P35240 Q8WUJ2 Q969Q3
Accessions AAD14139 AAF80764 CAA51220 CAA76992 CAA76993 EAW59814 EAW59815 EAW59816 EAW59817 EAW59818 EAW59819 EAW59820 EAW59821 EAW59822 EAW59823 EAW59824 EAW59825 EAW59826 EAW59827 EAW59828 EAW59829 EAW59830 EAW59831 EAW59832 EAW59833 P35240 AF113694 AAF29587 AF122827 AAD48752 AF122828 AAD48753 AF123570 AAD48754 AF369657 AAK54160 AF369658 AAK54161 AF369659 AF369660 AF369661 AAK54162 AF369662 AAK54163 AF369663 AAK54164 AF369664 AAK54165 AF369665 AAK54166 AF369666 AF369667 AF369668 AF369700 AAK54195 AF369701 AAK54196 AF369702 AF369703 AK092306 AK092515 AK297116 BAH12502 BC003112 AAH03112 BC007279 BC007336 BC010060 BC020257 AAH20257 BC071653 CF138178 CN419318 CR456530 CAG30416 CU013130 CAK54561 CU013418 CAK54860 DQ596361 DQ891697 ABM82623 DQ894878 ABM85804 EF517524 ABP73252 L11353 AAA36212 L27065 AAA62422 L27066 L28839 S73853 AAB31736 S73854 AAB31737 S73856 AAB31738 Z22664 CAA80377 NM_000268 NP_000259 NM_016418 NP_057502 NM_181825 NP_861546 NM_181828 NP_861966 NM_181829 NP_861967 NM_181830 NP_861968 NM_181831 NP_861969 NM_181832 NP_861970 NM_181833 NP_861971 NM_181826 NM_181827 NM_181834 NM_181835
Function Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. {ECO:0000269|PubMed:20159598, ECO:0000269|PubMed:20178741}.
Subcellular Location Isoform 9: Cytoplasm, perinuclear region. Cytoplasmic granule. Note=Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia.
Tissue Specificity Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
Top Pathways Hippo signaling pathway

Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb - 13281 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5A4I
Western blot analysis of extracts from PC-3 cells and wild-type (wt) and Nf2 knockout (-/-) mouse embryonic fibroblasts (MEFs), untreated (-) or λ phosphatase-treated (+), using Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb (upper), Merlin (D3S3W) Rabbit mAb #12888 (middle), or Ezrin/Radixin/Moesin Antibody #3142 (lower). (MEF wt and MEF Nf2 (-/-) cells were kindly provided by Dr. Andrea McClatchey, MGH Cancer Center and Harvard Medical School, Charlestown MA).

Phospho-Merlin (Ser518) Antibody - 9163 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC3 cells, untreated or treated with λ phosphatase, using Phospho-Merlin (Ser518) Antibody (upper) or Merlin Antibody #9168 (lower).

Merlin (D6N8H) Rabbit mAb - 12896 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6N8H
Western blot analysis of extracts from PC-3, PC-12, and C6 cells using Merlin (D6N8H) Rabbit mAb.

Merlin (D3S3W) Rabbit mAb - 12888 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3S3W
Western blot analysis of extracts from PC-3 cells, and both wild type and Nf2 (-/-) mouse embryonic fibroblasts (MEFs) using Merlin (D3S3W) Rabbit mAb (upper), Ezrin/Radixin/Moesin Antibody #3142 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wt and MEF Nf2 (-/-) cells were kindly provided by Dr. Andrea McClatchey and Dr. Andrew Gladden, MGH Cancer Center and Harvard Medical School, Charlestown MA).

Merlin (D1D8) Rabbit mAb - 6995 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1D8
Western blot analysis of extracts from fetal rat brain, and both wild type and NF2(-/-) mouse embryonic fibroblasts (MEFs) using Merlin (D1D8) Rabbit mAb (upper) and Ezrin/Radixin/Moesin Antibody #3142 (lower). (MEF wt and MEF NF2(-/-) cells were kindly provided by Dr. Andrea McClatchey and Dr. Andrew Gladden, MGH Cancer Center and Harvard Medical School, Charlestown MA).

Merlin (1C4) Mouse mAb - 9169 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 1C4

Merlin Antibody - 9168 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC3 and MRC-5 cells, using Merlin Antibody.

NF2 (B-12) - sc-55575 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
F2 (B-12): sc-55575. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

NF2 (E-2) - sc-55574 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NF2 (E-2): sc-55574. Western blot analysis of NF2 expression in MCF7 (A), CSMLO (B) and KNRK (C) whole cell lysates.

NF2 (H-260) - sc-28247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NF2 (H-260): sc-28247. Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.

NF2 (A-19) - sc-331 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NF2 (A-19): sc-331. Western blot analysis of NF2 expression in MCF7 (A) and Jurkat (B) whole cell lysates.

NF2 (C-18) - sc-332 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NF2 (C-18): sc-332. Western blot analysis of NF2 expression in KNRK whole cell lysate.

p-NF2 (Ser 518) - sc-101735 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-NF2 (Ser 518): sc-101735. Western blot analysis of phosphorylated NF2 expression in untreated (A) and IFN-α-treated (B) HUVEC whole cell lysates.

Anti-NF2 / Merlin (phospho S518) antibody - ab2478 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Rabbit polyclonal to phospho NF2 (Merlin) Ser 518 (ab2478) was used at a 1/1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2,3 and 4 respectively) for 45'. After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary antibody.

Anti-NF2 / Merlin (phospho S518) antibody - ab47378 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NF2 / Merlin (phospho S518) antibody (ab47378) at 1/500 dilutionLane 1 : HuvEc cell extracts treated with IFN-a(1000U/ml, 18 hours)Lane 2 : HuvEc cell extracts

Anti-NF2 / Merlin (phospho S518) antibody - ab131473 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NF2 / Merlin (phospho S518) antibody (ab131473) at 1/500 dilutionLane 1 : HUVEC cell extractLane 2 : HUVEC cell extract treated with IFN alpha

Anti-NF2 / Merlin (phospho S518) antibody - ab115687 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab115687 at 2.5ug/ml staining NF2/Merlin in Formalin-fixed, Paraffin-embedded Human brain (cortex) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-NF2 / Merlin antibody - ab30329 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NF2 / Merlin antibody (ab30329) at 1/1000 dilutionLane 1 : 3T3 cell lysateLane 2 : Cos7 cell lysateLane 3 : recombinant merlin proteinLane 4 : Normal human Schwann cell (NHSC)lysate

Anti-NF2 / Merlin antibody - ab70232 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic islet cell tumor tissue labelling NF2 / Merlin with ab70232 at 1/1000 (1µg/ml). Detection: DAB.

Anti-NF2 / Merlin antibody - ab84550 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of human NF2 / Merlin by Immunohistochemistry.Sample: formalin fixed, paraffin embedded section of human thyroid carcinoma.Antibody: ab84550 used at a dilution of 1/500.Detection: DAB staining.

Anti-NF2 / Merlin antibody - ab47502 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NF2 / Merlin antibody (ab47502) at 1/500 dilutionLane 1 : Extracts from HuvEc cells treated with IFN alpha (1000U/ml, 18 hours)Lane 2 : Extracts from HuvEc cells treated with IFN alpha (1000U/ml, 18 hours) and peptideLysates/proteins at 30 µg per lane.

Anti-NF2 / Merlin antibody [AF1G4] - ab88957 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AF1G4
All lanes : Anti-NF2 / Merlin antibody [AF1G4] (ab88957) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : MCF7 cell lysateLane 4 : HepG2 cell lysateLane 5 : MOLT4 cell lysateLane 6 : C6 cell lysateLane 7 : L929 cell lysate

Anti-NF2 / Merlin antibody [EPR2573(2)] - ab109244 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2573(2)
All lanes : Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244) at 1/50000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : Jurkat cell lysateLane 4 : PC3 cell lysateLysates/proteins at 10 µg per lane.

Anti-NF2 / Merlin antibody [mAbcam62839] - ab62839 from Abcam

Prices $384.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam62839
All lanes : Anti-NF2 / Merlin antibody [mAbcam62839] (ab62839) at 1/200 dilutionLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NF2 / Merlin Antibody (phospho-Ser518) - LS-C8995 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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