![All lanes : Anti-NF2 / Merlin antibody [AF1G4] (ab88957) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : MCF7 cell lysateLane 4 : HepG2 cell lysateLane 5 : MOLT4 cell lysateLane 6 : C6 cell lysateLane 7 : L929 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29805_NF2-Merlin-Primary-antibodies-ab88957-2.jpg)
All lanes : Anti-NF2 / Merlin antibody [AF1G4] (ab88957) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : MCF7 cell lysateLane 4 : HepG2 cell lysateLane 5 : MOLT4 cell lysateLane 6 : C6 cell lysateLane 7 : L929 cell lysate
ICC/IF image of ab88957 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88957, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab88957, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of NF2/Merlin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88957, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.