Anti-NF2 / Merlin antibody [mAbcam62839]

• Supplier: Abcam
• Catalog: ab62839
• Prices: $384.00
• Sizes: 500 µl
• Host: Mouse
• Clonality: Monoclonal
• Clone: mAbcam62839
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant fragment derived from Human NF2/ Merlin
• Description: Mouse Monoclonal
• Gene: NF2
• Conjugate: Unconjugated

Product images

All lanes : Anti-NF2 / Merlin antibody [mAbcam62839] (ab62839) at 1/200 dilutionLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab62839 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62839 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing HeLa cells stained with ab62839 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62839, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.