Anti-NFATC4 antibody
| Name | Anti-NFATC4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62613 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | A synthesized non-phosphopeptide derived from human NFATC4 around the phosphorylation site of serine 676 |
| Description | Rabbit Polyclonal |
| Gene | NFATC4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence analysis of HeLa cells, using ab62613 at a 1:500 dilution.Left image untreated.Right image treated with peptide.
Ab62613 staining human normal colon tissue. Staining is localised to subcellular compartment.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Recombination signal binding protein for Ig-kappaJ region regulates - Recombination signal binding protein for Ig-kappaJ region regulates
Castellanos-Rivera RM, Pentz ES, Lin E, Gross KW, Medrano S, Yu J, Sequeira-Lopez ML, Gomez RA. J Am Soc Nephrol. 2015 Jan;26(1):67-80.
Sensitization of ethanol-induced place preference as a result of up-regulation of - Sensitization of ethanol-induced place preference as a result of up-regulation of
Kurokawa K, Mizuno K, Ohkuma S. J Neurochem. 2014 Dec;131(6):836-47.
Genes that confer the identity of the renin cell. - Genes that confer the identity of the renin cell.
Brunskill EW, Sequeira-Lopez ML, Pentz ES, Lin E, Yu J, Aronow BJ, Potter SS, Gomez RA. J Am Soc Nephrol. 2011 Dec;22(12):2213-25.
Cognitive decline in Alzheimer's disease is associated with selective changes in - Cognitive decline in Alzheimer's disease is associated with selective changes in
Abdul HM, Sama MA, Furman JL, Mathis DM, Beckett TL, Weidner AM, Patel ES, Baig I, Murphy MP, LeVine H 3rd, Kraner SD, Norris CM. J Neurosci. 2009 Oct 14;29(41):12957-69.