Anti-NFkB p100 / p52 antibody - ChIP Grade
| Name | Anti-NFkB p100 / p52 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab7972 |
| Prices | $385.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IP WB ICC/IF ICC/IF ChIP EMSA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: VFLQLKRKRGGDVSDSKQFT , corresponding to C terminal amino acids 305-324 of Mouse NFkB p100/ p52(Peptide available as ab8007 |
| Description | Rabbit Polyclonal |
| Gene | NFKB2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab7972 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7972 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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