Anti-NFYA antibody [EPR9061]

• Supplier: Abcam
• Catalog: ab139402
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9061
• Applications: WB ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)
• Description: Rabbit Monoclonal
• Gene: NFYA
• Conjugate: Unconjugated

Product images

ab139402 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139402 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-NFYA antibody [EPR9061] (ab139402) at 1/1000 dilutionLane 1 : NIH 3T3 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Flow Cytometrical analysis of permeabilized NIH 3T3 cells labelling NFYA with ab139402 at 1/10 dilution (red) or a rabbit IgG (negative) (green).