NFYA

Gene Symbol NFYA
Entrez Gene 4800
Alt Symbol CBF-A, CBF-B, HAP2, NF-YA
Species Human
Gene Type protein-coding
Description nuclear transcription factor Y, alpha
Other Description CAAT box DNA-binding protein subunit A|CAAT-box DNA binding protein subunit A|CCAAT-binding transcription factor subunit B|HAP2 CCAAT-binding protein|Transcription factor NF-Y, A subunit|nuclear transcription factor Y subunit A|nuclear transcription factor Y subunit alpha
Swissprots Q8IXU0 P23511
Accessions EAX04013 EAX04014 EAX04015 EAX04016 EAX04017 EAX04018 EAX04019 EAX04020 EAX04021 P23511 AA905268 AJ420494 AK002098 AK025201 BC026923 BC039244 AAH39244 BM561405 BQ184804 EU446898 ABZ92427 GQ167198 ADD81252 M59079 AAA35950 X59711 CAA42231 NM_002505 NP_002496 NM_021705 NP_068351
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'- CCAAT-3' box motif found in the promoters of its target genes. NF- Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1. {ECO:0000269|PubMed:12741956}.
Subcellular Location Nucleus.
Top Pathways Tuberculosis, Antigen processing and presentation

NF-YA (G-2) - sc-17753 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NF-YA (G-2): sc-17753. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

NF-YA (H-209) - sc-10779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NF-YA (H-209): sc-10779. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.

NF-YA (G-15) - sc-30907 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NF-YA (G-15): sc-30907. Western blot analysis of NF-YA expression in K-562 whole cell lysate.

NF-YA (Y-12) - sc-30906 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NF-YA (Y-12): sc-30906. Western blot analysis of NF-YA expression in A-431 whole cell lysate.

NF-YA (C-18) - sc-7712 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NF-YA (C-18): sc-7712. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of cells in germinal and non-germinal centers.

Anti-NFYA antibody - ab6558 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NFYA antibody (ab6558) at 1/500 dilution + CaPan1 whole cell lysate at 50 µgSecondaryHRP-conjugated Donkey anti-Rabbit. at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NFYA antibody - ab154554 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NFYA antibody (ab154554) at 1/3000 dilutionLane 1 : NIH 3T3 whole cell lysateLane 2 : JC whole cell lysateLane 3 : BCL1 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-NFYA antibody - ab22886 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-NFYA antibody - ab19462 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-NFYA antibody - ab64806 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NFYA antibody (ab64806) at 1/500 dilutionLane 1 : Extracts of 293 cellsLane 2 : Extracts of HepG2 cellsLane 3 : Extracts of COLO205 cellsLane 4 : Extracts of COLO205 cells plus peptideLysates/proteins at 5 µg per lane.

Anti-NFYA antibody [EPR9061] - ab139402 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9061
ab139402 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139402 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NFYA antibody - N-terminal - ab189130 from Abcam

Prices $379.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling NFYA with ab189130 at 1/400 dilution, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-NFYA Antibody (aa333-347) - LS-C288861 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse NF-YA by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-NF-YA used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-NFYA Antibody (C-Terminus) - LS-C29688 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NFYA antibody LS-C29688 was used in IHC to stain formalin-fixed, paraffin-embedded human muscle.

Anti-NFYA Antibody (aa261-310) - LS-C118829 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HepG2/293/COLO205 cells, using NFYA Antibody. The lane on the right is treated with the synthesized peptide.

Anti-NFYA Antibody (aa320-333) - LS-C288862 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human NF-YA by Immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-NF-YA used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-NFYA Antibody (aa333-347) - LS-C286785 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse NF-YA by Immunohistochemistry. Sample: FFPE section of mouse hybridoma tumor. Antibody: Affinity purified rabbit anti-NF-YA used at a dilution of 1:250.

Anti-NFYA Antibody (N-Terminus) IHC-plus™ - LS-B131 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.

Anti-NFYA Antibody (N-Terminus) - LS-C9324 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NFYA Antibody (N-Terminus) - LS-C9327 from LifeSpan Bioscience

Prices $635.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NFYA Antibody (N-Terminus) - LS-C73808 from LifeSpan Bioscience

Prices $530.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-NFYA Antibody (Lys290) - LS-C191950 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NFYA Antibody (C-Terminus) - LS-C31566 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
NFYA antibody LS-C31566 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.

NFYA Antibody - NBP2-19533 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000.

NFYA Antibody - NB600-481 from Novus Biologicals

Prices $369.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
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