Anti-NGF antibody
| Name | Anti-NGF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab6199 |
| Prices | $400.00 |
| Sizes | 250 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF DB B/N WB IHC-F IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Chicken, Human |
| Antigen | Native mouse salivary gland NGF purified by modification of the method of Mobley et al (1976) |
| Description | Rabbit Polyclonal |
| Gene | NGF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab6199 at a 1/500 dilution staining rat brain tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue section was paraformaldehyde fixed and blocked with 2% BSA prior to incubation with the antibody for 24 hours. Bound antibody was detected using a biotinylated goat anti-rabbit IgG antibody. This image is courtesy of an Abreview submitted by Grazyna Niewiadomska.See Abreview
ab6199 staining perfusion fixed rat brain and dorsal root ganglion by IHC-Fr. Animals were pre-perfused with Tris buffer pH 10, followed by 4% paraformadehyde and 15% of a saturated solution of picric acid. The brains were post-fixed in the same fixative overnight, cryoprotected in 20% sucrose for 24 hours, frozen and cut with a cryostat. Free floating immunostaining was performed. An Alexa Fluor ® 488 conjugated goat anti-rat antibody was used as the secondary.The image shows the staining obtained with this antibody using direct fluorescence in the rat cortex and dorsal root ganglion. The staining is not only of the cell body of the cortical neurons but a part of their processes.See Abreview
ICC/IF image of ab6199 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6199, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-NGF antibody (ab6199) at 1 µg/ml + Active human NGF full length protein (ab69759) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
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