All lanes : Anti-NGF antibody (ab66459) at 1 µg/mlLane 1 : Lung (Human) Tissue Lysate - adult normal tissueLane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
All lanes : Anti-NGF antibody (ab66459) at 1 µg/mlLane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor cell line (ab30124)Lane 2 : Liver (Mouse) Tissue LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 5 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)Lane 6 : Thymus (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab66459 stained SHSY5Y cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.