Anti-NGF antibody [EP1318Y]

• Supplier: Abcam
• Catalog: ab68151
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1318Y
• Applications: WB FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues near the N-terminus of human NGF-beta precursor (pro-NGF)
• Description: Rabbit Monoclonal
• Gene: NGF
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)Lane 1 : 10ng human pro-NGF recombinant proteinLane 2 : 5ng human pro-NGF recombinant proteinLane 3 : 1ng human pro-NGF recombinant proteinLane 4 : 0.5ng human pro-NGF recombinant proteinSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution