All lanes : Anti-NHP2 antibody [EPR12404] (ab180498) at 1/2000 dilutionLane 1 : K562 lysateLane 2 : Jurkat lysateLane 3 : Caco 2 lysateLane 4 : SH-SY5Y lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NHP2 with ab180498 at 1/500 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG and counter stained with Hematoxylin.
Flow Cytometric analysis of K562 cells (2% paraformaldehyde-fixed) labeling NHP2 with ab180498 at 1/20 (red) compared to a Rabbit monoclonal IgG negative control antibody (green) followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.