Western blot analysis of total SW480 cell lysates using Non-phospho (Active) β-Catenin (Ser33/Ser37/Thr41) Antibody in the presence of a non-phospho-β-catenin peptide (Lane 4) and various peptides phosphorylated at different positions, including phospho-β-catenin (Ser33) peptide (Lane 1), phospho-β-catenin (Ser33/37) peptide (Lane 2), phospho-β-catenin (Ser33/37/Thr41) peptide (Lane 3), phospho-β-catenin (Ser37) peptide (Lane 5), phospho-β-catenin (Ser37/Thr41) peptide (Lane 6), and phospho-β-catenin (Thr41) peptide (Lane 7).
Western blot analysis of SW480 cells treated with Calyculin A #9902 and immunoprecipitated using Phospho-β-Catenin (Ser33/37) Antibody #2009 (Lane 2), β-Catenin Antibody (Amino-terminal Antigen) #9581 (Lane 3) and an unrelated rabbit IgG as negative control (Lane 4). The immunoprecipitates along with the input lysate (Lane 1) were subject to western analysis using β-Catenin (6B3) Rabbit mAb #9582 (upper), Phospho-β-Catenin (Ser33/37) Antibody #2009 (middle), and