Overlay histogram showing SHSY-5Y cells stained with ab76159 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76159, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
![Anti-Nicotinic Acetylcholine Receptor beta antibody [EP2067Y] (ab76159) at 1/1000 dilution + human brain lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/318_ab76159wb.gif)
Anti-Nicotinic Acetylcholine Receptor beta antibody [EP2067Y] (ab76159) at 1/1000 dilution + human brain lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution