Anti-Ninein antibody - Centrosome Marker
| Name | Anti-Ninein antibody - Centrosome Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab4447 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse |
| Antigen | Fusion protein containing amino acids 32-506 of murine Ninein |
| Description | Rabbit Polyclonal |
| Gene | NIN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IF using ab4447 to detect Ninein in MCF7 cells (human).The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein.ab4447 was used at 1/1000.Ninein shows nuclear and centrosome staining, which is expected from the literature.
ICC/IF image of ab4447 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4447, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red). ab4447 shows the characteristic nuclear and centrosome staining for Ninein.
Product References
Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not - Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not
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Abnormal centrosomal structure and duplication in Cep135-deficient vertebrate - Abnormal centrosomal structure and duplication in Cep135-deficient vertebrate
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LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein - LIS1 controls mitosis and mitotic spindle organization via the LIS1-NDEL1-dynein
Moon HM, Youn YH, Pemble H, Yingling J, Wittmann T, Wynshaw-Boris A. Hum Mol Genet. 2014 Jan 15;23(2):449-66.
STIL is required for centriole duplication in human cells. - STIL is required for centriole duplication in human cells.
Vulprecht J, David A, Tibelius A, Castiel A, Konotop G, Liu F, Bestvater F, Raab MS, Zentgraf H, Izraeli S, Kramer A. J Cell Sci. 2012 Mar 1;125(Pt 5):1353-62.
Cep57, a NEDD1-binding pericentriolar material component, is essential for - Cep57, a NEDD1-binding pericentriolar material component, is essential for
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Defective nucleotide excision repair with normal centrosome structures and - Defective nucleotide excision repair with normal centrosome structures and
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The human IFN-inducible p53 target gene TRIM22 colocalizes with the centrosome - The human IFN-inducible p53 target gene TRIM22 colocalizes with the centrosome
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CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage - CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage
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Murine CENP-F regulates centrosomal microtubule nucleation and interacts with - Murine CENP-F regulates centrosomal microtubule nucleation and interacts with
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Ste20-related protein kinase LOSK (SLK) controls microtubule radial array in - Ste20-related protein kinase LOSK (SLK) controls microtubule radial array in
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