NIN

Gene Symbol NIN
Entrez Gene 51199
Alt Symbol SCKL7
Species Human
Gene Type protein-coding
Description ninein (GSK3B interacting protein)
Other Description glycogen synthase kinase 3 beta-interacting protein|hNinein|ninein|ninein centrosomal protein
Swissprots Q9HAT7 Q9UH61 C9JSG2 Q5BKU3 Q8N4C6 Q6P0P6 Q9H5I6 Q9BWU6 C9JXL2 Q9HCK7 C9JSB6 A6NDB8 Q9C014 Q9C013 Q9C012 Q9HBY5 B7WPA3
Accessions AAV37180 EAW65692 EAW65693 EAW65694 EAW65695 EAW65696 EAW65697 EAW65698 EAW65699 EAW65700 EAW65701 Q8N4C6 AA742651 AB046785 BAB13391 AF186776 AAG17027 AF212162 AAF23015 AF223937 AAK00628 AF223938 AAK00629 AF223939 AAK00630 AF302773 AAG33512 AK025652 AK026083 AK027054 BAB15640 AL359571 AW505195 AY027794 AAK27375 AY027795 AAK27376 AY027796 AAK27377 AY736182 AAU86898 AY736183 AAU86899 BC034708 AAH34708 BC065521 AAH65521 BC090932 AAH90932 BM721510 CB241926 CR993064 HG506189 XM_005267735 XP_005267792 XM_006720160 XP_006720223 XM_011536817 XP_011535119 XM_011536818 XP_011535120 XM_011536819 XP_011535121 XM_011536820 XP_011535122 XM_011536821 XP_011535123 XM_011536822 XP_011535124 XM_011536823 XP_011535125 XM_011536824 XP_011535126 NM_016350 NP_057434 NM_020921 NP_065972 NM_182944 NP_891989 NM_182946 NP_891991 NM_182945
Function Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. {ECO:0000269|PubMed:15190203}.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:11004522, ECO:0000269|PubMed:11956314, ECO:0000269|PubMed:12403812, ECO:0000269|PubMed:12927815, ECO:0000269|PubMed:15190203}. Note=Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization.
Tissue Specificity Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. {ECO:0000269|PubMed:11004522, ECO:0000269|PubMed:11162463}.

Ninein (F-5) - sc-376420 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ninein (F-5): sc-376420. Western blot analysis of Ninein expression in rat skeletal muscle tissue extract.

Ninein (H-188) - sc-292089 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Ninein (Y-16) - sc-50142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ninein (Y-16): sc-50142. Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.

Anti-Ninein antibody - ab176803 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ninein antibody (ab176803) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH 3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-Ninein antibody - Aminoterminal end - ab52473 from Abcam

Prices $387.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ninein antibody - Aminoterminal end (ab52473) at 1/500 dilutionLane 1 : MCF7 cell line extractLane 2 : MOLT4 cell line extractSecondaryHRP conjugated donkey anti-rabbit developed using the ECL techniqueObserved band size : 200 kDa (why is the actual band size different from the predicted?)Additional bands at : 110 kDa,130 kDa. We are unsure as to the identity of these extra bands.

Anti-Ninein antibody - Centrosome Marker - ab4447 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IF using ab4447 to detect Ninein in MCF7 cells (human).The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein.ab4447 was used at  1/1000.Ninein shows nuclear and centrosome staining, which is expected from the literature.

Anti-Ninein antibody - N-terminal - ab200165 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ninein antibody - N-terminal (ab200165) at 1/1000 dilutionLane 1 : Total cell extracts from mouse thymusLane 2 : Total cell extracts from Human Jurkat

Anti-NIN / Ninein Antibody (aa1850-1900) - LS-C288296 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIN / Ninein Antibody (N-Terminus) - LS-C41207 from LifeSpan Bioscience

Prices $205.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
MCF7 (Lane 1) and MOLT-4 (Lane 2) cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-ninein antibody.

Ninein Antibody - NB100-74631 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Ninein Antibody - NBP2-13657 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-GLRB Polyclonal Antibody - bs-11559R from Bioss

Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti GLRB Polyclonal Antibody, Unconjugated (bs-11559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

GSK3B interacting protein Antibody - bs-7844R from Bioss

Prices $279.50
Sizes 100 µl
Host Rabbit
Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti Ninein/GSK3B interacting protein Polyclonal Antibody, Unconjugated (bs-7844R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Rabbit Anti-GLRB Polyclonal Antibody, Cy3 Conjugated - bs-11559R-Cy3 from Bioss

Host Rabbit
Clonality Polyclonal

Rabbit Anti-GLRB Polyclonal Antibody, Cy5 Conjugated - bs-11559R-Cy5 from Bioss

Host Rabbit
Clonality Polyclonal

Rabbit Anti-GLRB Polyclonal Antibody, Cy7 Conjugated - bs-11559R-Cy7 from Bioss

Host Rabbit
Clonality Polyclonal

Rabbit Anti-GLRB Polyclonal Antibody, HRP Conjugated - bs-11559R-HRP from Bioss

Host Rabbit
Clonality Polyclonal

Anti-NIN antibody produced in rabbit - HPA005939 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NIN:<B>Cat. No. HPA005939</B>: Immunohistochemical staining of human appendix shows distinct dot-like positivity in lymphoid cells.
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