Anti-NIRF antibody (ab28673) at 0.5 µg/ml + Jurkat Cell Lysate with 5% skim milk / PBS bufferSecondaryHRP conjugated anti-Rabbit IgG1: 50,000 - 100,000 dilution.Observed band size : 90 kDa (why is the actual band size different from the predicted?)Gel concentration: 12%
IHC staining of paraffin embedded human heart with ab28673 at 4.0-8.0 ug/ml. Positive staining (arrows) is myocardial cells. Magnification: x400
developed using the ECL techniquePerformed under reducing conditions.Exposure time : 2 minutesThis image is courtesy of an anonymous AbreviewWestern blot analysis using ab28673 at 1/2000.Lane 1) Whole cell HeLa lysateLane 2) Recombinant purified UHRF2 proteinLane 3) Recombinant purified UHRF1 proteinLane 4) Whole cell HeLa lysate with UHRF1 knockdown (short hairpin RNA)A HRP-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview