UHRF2

Gene Symbol UHRF2
Entrez Gene 115426
Alt Symbol NIRF, RNF107, URF2
Species Human
Gene Type protein-coding
Description ubiquitin-like with PHD and ring finger domains 2, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase UHRF2|Np95-like ring finger protein|RING finger protein 107|np95/ICBP90-like RING finger protein|nuclear protein 97|nuclear zinc finger protein NP97|ubiquitin-like PHD and RING finger domain-containing protein 2|ubiquitin-like, containing PHD and RING finger domains, 2|ubiquitin-like-containing PHD and RING finger domains protein 2
Swissprots Q5VYR1 Q659C8 Q5VYR3 Q96PU4 Q8TAG7
Accessions EAW58743 Q96PU4 AB071698 BAB68317 AF274049 AAM33799 AK122730 BAG53694 AK308433 AL137728 CAH56383 AL833522 CAH10389 BC028397 AAH28397 BG709183 EU176286 ABW03737 EU176602 ABW03403 XM_011517703 XP_011516005 XM_011517704 XP_011516006 XM_011517705 XP_011516007 XM_011517706 XP_011516008 XR_428418 XR_428419 XR_428420 XR_428421 XR_929173 NM_152896 NP_690856 NR_046386
Function E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. E3 SUMO-, but not ubiquitin-, protein ligase for ZNF131. {ECO:0000269|PubMed:12176013, ECO:0000269|PubMed:14741369, ECO:0000269|PubMed:15178429, ECO:0000269|PubMed:15361834, ECO:0000269|PubMed:21952639, ECO:0000269|PubMed:23404503}.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00358, ECO:0000269|PubMed:12176013, ECO:0000269|PubMed:23404503}. Note=Enriched at pericentric heterochromatin (PH). This localization is dependent on the interaction with H3K9me3 (By similarity). {ECO:0000250}.

NIRF (C-10) - sc-398953 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NIRF (C-10): sc-398953. Western blot analysis of NIRF expression in Jurkat whole cell lysate (A) and Jurkat (B), HeLa (C) and SK-N-MC (D) nuclear extracts.

NIRF (L-16) - sc-54248 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NIRF (T-18) - sc-54252 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NIRF (T-18): sc-54252. Western blot analysis of NIRF expression in non-transfected: sc-110760 (A) and human NIRF transfected: sc-170276 (B) 293 whole cell lysates.

NIRF (L-22) - sc-133822 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
NIRF (L-22): sc-133822. Western blot analysis of NIRF expression in Jurkat whole cell lysate.

NIRF (N-17) - sc-54249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NIRF (N-17): sc-54249. Western blot analysis of NIRF expression in K-562 (A), Hep G2 (B), F9 (C) and CHO-K1 (D) whole cell lysates.

Anti-NIRF antibody - ab28673 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NIRF antibody (ab28673) at 0.5 µg/ml + Jurkat Cell Lysate with 5% skim milk / PBS bufferSecondaryHRP conjugated anti-Rabbit IgG1: 50,000 - 100,000 dilution.Observed band size : 90 kDa (why is the actual band size different from the predicted?)Gel concentration: 12%

Anti-NIRF antibody - ab22235 from Abcam

Prices $359.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-NIRF antibody (ab22235) at 1/1000 dilutionLane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ugLane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug SecondaryRabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Anti-NIRF antibody - ab57973 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57973 NIRF antibody at 1ug/ml.

Anti-NIRF antibody - ab116078 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NIRF antibody (ab116078) at 1 µg/ml + Mouse pancreas lysate at 10 µg

Anti-NIRF / UHRF2 Antibody (aa479-508, Biotin) - LS-C273030 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIRF / UHRF2 Antibody (aa479-508, Azide-free, HRP) - LS-C273031 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIRF / UHRF2 Antibody (aa479-508, FITC) - LS-C273032 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIRF / UHRF2 Antibody (aa479-508, PE) - LS-C273033 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIRF / UHRF2 Antibody (aa478-508) - LS-C326182 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIRF / UHRF2 Antibody (aa540-589) - LS-C109942 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UHRF2 antibody LS-C109942 Western blot of Fetal liver lysate.

Anti-NIRF / UHRF2 Antibody - LS-C341308 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NIRF / UHRF2 Antibody (aa186-235) - LS-C29893 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UHRF2 antibody LS-C29893 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.

Anti-NIRF / UHRF2 Antibody (aa479-508) - LS-C157322 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
UHRF2 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The UHRF2 antibody detected the UHRF2 protein (arrow).

Anti-NIRF / UHRF2 Antibody - LS-C332076 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using UHRF2 antibody.

Anti-NIRF / UHRF2 Antibody - LS-C305930 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of NIRF / UHRF2 antibody LS-X223182.

Anti-NIRF / UHRF2 Antibody (aa24-73) IHC-plus™ - LS-B9657 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NIRF / UHRF2 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9657 concentration 5 ug/ml.

Anti-NIRF / UHRF2 Antibody (aa479-508, APC) - LS-C273029 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIRF / UHRF2 Antibody (aa479-508, AP) - LS-C273028 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

NIRF Antibody - NBP1-92182 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NIRF Antibody [NBP1-92182] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4

NIRF Antibody - NBP1-92183 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: NIRF Antibody [NBP1-92183] Staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
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