![All lanes : Anti-NIT1 antibody [EPR14577(B)] (ab180942) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : Fetal liver lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/489_ab180942-212409-ab180942.jpg)
All lanes : Anti-NIT1 antibody [EPR14577(B)] (ab180942) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : Fetal liver lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NIT1 with ab180942 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot analysis of fetal liver lysate immunoprecipitated using ab180942 at 1/60 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.