NIT1

Gene Symbol	NIT1
Entrez Gene	4817
Alt Symbol	-
Species	Human
Gene Type	protein-coding
Description	nitrilase 1
Other Description	nitrilase homolog 1
Swissprots	D3DVF4 B1AQP3 O76091 Q86X76
Accessions	AAC39901 EAW52654 EAW52655 EAW52656 EAW52657 Q86X76 AF069987 AAC39907 AI277603 AK296606 BAH12396 AK313323 BAG36128 AY893504 AAX36541 AY893938 AAX36975 BC046149 AAH46149 BI752623 BQ002524 BX416745 CB111935 CR541814 CAG46613 CR541846 CAG46644 DC302367 XM_005245214 XP_005245271 XM_005245215 XP_005245272 XM_005245216 XP_005245273 XM_006711341 XP_006711404 NM_001185092 NP_001172021 NM_001185093 NP_001172022 NM_001185094 NP_001172023 NM_005600 NP_005591
Function	Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2 (By similarity). {ECO:0000250}.
Subcellular Location	Cytoplasm. Mitochondrion {ECO:0000250}.
Tissue Specificity	Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. {ECO:0000269\|PubMed:9671749}.

NIT1 (P-12) - sc-160079 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NIT1 (P-12): sc-160079. Western blot analysis of NIT1 expression in HeLa whole cell lysate.

Anti-NIT1 antibody - ab198203 from Abcam

Prices	$382.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NIT1 antibody (ab198203) at 1/250 dilutionLane 1 : Human placenta tissue lysateLane 2 : Human fetal liver tissue lysateLysates/proteins at 40 µg per lane.

Anti-NIT1 antibody - ab98066 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 antibody (ab98066) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T Cell Lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-NIT1 antibody [4D12] - ab118158 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4D12

All lanes : Anti-NIT1 antibody [4D12] (ab118158) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NIT1 cDNALysates/proteins at 5 µg per lane.

Anti-NIT1 antibody [EPR14577(B)] - ab180942 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR14577(B)

All lanes : Anti-NIT1 antibody [EPR14577(B)] (ab180942) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : Fetal liver lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-NIT1 antibody - C-terminal - ab171437 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 antibody - C-terminal (ab171437) at 1/100 dilution + ZR-75-1 cell line lysate at 35 µg

Anti-NIT1 Antibody (clone 4D12) - LS-C115078 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4D12

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).

Anti-NIT1 Antibody (clone 1C3) - LS-C197785 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1C3

Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]

Anti-NIT1 Antibody (clone 3A11) - LS-C115125 from LifeSpan Bioscience

Prices	$350.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3A11

IHC of paraffin-embedded Kidney tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).

Anti-NIT1 Antibody (aa298-327, Biotin) - LS-C264403 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 Antibody (aa298-327, AP) - LS-C264401 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 Antibody (aa298-327, APC) - LS-C264404 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 Antibody (aa298-327, FITC) - LS-C264402 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 Antibody (aa298-327, Azide-free, HRP) - LS-C264405 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 Antibody (aa298-327, PE) - LS-C264406 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 Antibody (aa297-327) - LS-C323339 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1 Antibody (aa71-120) - LS-C107914 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

NIT1 antibody LS-C107914 Western blot of Fetal Small Intestine lysate.

Anti-NIT1 Antibody (aa71-120) - LS-C107704 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

NIT1 antibody LS-C107704 Western blot of 293T cell lysate.

Anti-NIT1 Antibody (aa298-327) - LS-C164881 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

NIT1 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The NIT1 antibody detected the NIT1 protein (arrow).

Nitrilase 1 Antibody (3A11) - NBP2-02462 from Novus Biologicals

Prices	$379.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3A11

Western Blot: Nitrilase 1 Antibody (3A11) [NBP2-02462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nitrilase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nitrilase 1.

Nitrilase 1 Antibody - NBP1-87481 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Nitrilase 1 Antibody (1C3) - H00004817-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1C3

Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.

NIT1 Polyclonal Antibody - bs-6077R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NIT1, (N-terminal) antibody produced in rabbit - SAB2104899 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-NIT1, antibody produced in rabbit: <B>Cat. No. SAB2104899</B>: Immunoblotting analysis of NIT1 in 293T cell lysate with NIT1 anitbody at 1 μg/mL.

Anti-NIT1 antibody produced in rabbit - HPA006657 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-NIT1: <B>Cat. No. HPA006657</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of cells in exocrine pancreas and an additional granular staining pattern in islet cells.

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