Anti-NLK antibody
| Name | Anti-NLK antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab26050 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Chicken, Dog, Human, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human NLK |
| Description | Rabbit Polyclonal |
| Gene | NLK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-NLK was used in standard Vector ABC protocol on paraffin embedded mouse lung with the following modifications: HIER with Vector antigen unmasking solution for 25 minutes at 98C. Used a 1:100 dilution in PBS/Triton-X 4C overnight.
Adult mouse brain fixed in 10% neutral buffered formulin. Antigen retrieval was performed using Tris-EDTA pH 9.0. The tissue was incubated with ab26050 at a concentration of 2.5µg/ml for 16 hours at 4°C. Nuclear staining can be clearly seen.
ICC/IF image of ab26050 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26050 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.