NLK

Gene Symbol NLK
Entrez Gene 51701
Alt Symbol -
Species Human
Gene Type protein-coding
Description nemo-like kinase
Other Description serine/threonine-protein kinase NLK
Swissprots Q2PNI9 Q6P2A3 B2RCX1 Q9UBE8
Accessions ABC40748 EAW51060 EAW51061 EAW51062 EAW51063 Q9UBE8 AF180819 AAD56013 AF197898 AAF04857 AK024686 AK315315 BAG37718 AL036279 AL161970 BC064663 AAH64663 BM723604 BQ636970 DA224552 DA573077 U69562 XM_005257988 XP_005258045 XM_011524888 XP_011523190 XR_934482 NM_016231 NP_057315
Function Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin tar
Subcellular Location Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity). {ECO:0000250}.
Top Pathways Wnt signaling pathway, Adherens junction, MAPK signaling pathway

Nlk (H-2) - sc-271323 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nlk (H-2): sc-271323. Western blot analysis of Nlk expression in rat brain tissue extract.

Nlk (H-100) - sc-28884 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nlk (H-100): sc-28884. Western blot analysis of human recombinant Nlk.

Nlk (B-5) - sc-48361 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nlk (B-5): sc-48361. Western blot analysis of human recombinant Nlk.

Nlk (C-20) - sc-8210 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nlk (C-20): sc-8210. Western blot analysis of human recombinant Nlk.

Anti-NLK antibody - ab26050 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NLK was used in standard Vector ABC protocol on paraffin embedded mouse lung with the following modifications: HIER with Vector antigen unmasking solution for 25 minutes at 98C. Used a 1:100 dilution in PBS/Triton-X 4C overnight.

Anti-NLK antibody - ab97642 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NLK antibody (ab97642) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-NLK antibody - ab116715 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NLK antibody (ab116715) at 1/100 dilution + MCF7 cell line lysate at 35 µg

Anti-NLK antibody - ab57208 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57208 NLK antibody at 1ug/ml.

Anti-NLK antibody [EPR2012(2)] - ab154199 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2012(2)
All lanes : Anti-NLK antibody [EPR2012(2)] (ab154199) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : A375 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution

Anti-NLK antibody - C-terminal - ab157178 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-NLK antibody - C-terminal (ab157178) at 1/1000 dilution + Mouse brain total cell extract

Anti-NLK Antibody (aa275-300, AP) - LS-C264478 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa275-300, APC) - LS-C264479 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa275-300, Biotin) - LS-C264480 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa275-300, FITC) - LS-C264482 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa275-300, Azide-free, HRP) - LS-C264481 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa275-300, PE) - LS-C264483 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa407-436, AP) - LS-C264484 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa407-436, Biotin) - LS-C264486 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa431-480) - LS-C117249 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NLK antibody LS-C117249 Western blot of 293T cell lysate.

Anti-NLK Antibody (aa407-436, APC) - LS-C264485 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa407-436, Azide-free, HRP) - LS-C264488 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa407-436, PE) - LS-C264491 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (C-Terminus, AP) - LS-C280141 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (C-Terminus, APC) - LS-C280142 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NLK Antibody (aa407-436, FITC) - LS-C264487 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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