Anti-NMDAR1 antibody - Neuronal Marker
| Name | Anti-NMDAR1 antibody - Neuronal Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab17345 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Rat, Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat NMDAR1 |
| Blocking Peptide | Rat NMDAR1 peptide |
| Description | Rabbit Polyclonal |
| Gene | GRIN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5µg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.
Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml + HEK293 cell lysate co-transfected with NR1 and NR2A recombinant protein at 50 µg
All lanes : Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/mlLane 1 : HEK293 Whole Cell Lysate Transfected with SHAMLane 2 : HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2ALysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Product References
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