All lanes : Anti-Nmnat1 antibody (ab45548) at 1/250 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.