NMNAT1

Gene Symbol NMNAT1
Entrez Gene 64802
Alt Symbol LCA9, NMNAT, PNAT1
Species Human
Gene Type protein-coding
Description nicotinamide nucleotide adenylyltransferase 1
Other Description NMN adenylyltransferase 1|NMN/NaMN adenylyltransferase 1|NaMN adenylyltransferase 1|nicotinamide mononucleotide adenylyltransferase 1|nicotinamide-nucleotide adenylyltransferase 1|nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1|nicotinate-nucleotide adenylyltransferase 1|pyridine nucleotide adenylyltransferase 1
Swissprots Q9H247 Q9HAN9 Q9H6B6 Q8TAE9 B1AN63
Accessions AAL76935 CAK32267 CAM12697 CDH92521 EAW71635 EAW71636 Q9HAN9 AF088049 AF312734 AAG33629 AF314163 AAG33632 AF459819 AAL76934 AI247931 AK026065 BAB15345 AK222920 BAD96640 AK315640 BAG38007 BC014943 AAH14943 BC032483 BG751629 BP309588 BP339298 BU948893 HG491681 JF432503 ADZ15720 XM_011541971 XP_011540273 NM_001297778 NP_001284707 NM_001297779 NP_001284708 NM_022787 NP_073624
Function Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following mechanical or toxic insults. {ECO:0000269|PubMed:17402747}.
Subcellular Location Nucleus {ECO:0000269|PubMed:11248244, ECO:0000269|PubMed:12574164, ECO:0000269|PubMed:16118205, ECO:0000269|PubMed:22842230}.
Tissue Specificity Widely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain. {ECO:0000269|PubMed:11027696, ECO:0000269|PubMed:11891043}.
Top Pathways Nicotinate and nicotinamide metabolism

NMNAT-1 (B-7) - sc-271557 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NMNAT-1 (B-7): sc-271557. Western blot analysis of NMNAT-1 expression in HeLa nuclear extract.

NMNAT-1 (D-20) - sc-30842 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NMNAT-1 (N-17) - sc-30841 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NMNAT-1 (N-17): sc-30841. Western blot analysis of NMNAT-3 expression in non-transfected: sc-117752 (A) and mouse transfected: sc-122083 (B) 293T whole cell lysates.

NMNAT-1 (H-109) - sc-98249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NMNAT-1 (H-109): sc-98249. Western blot analysis of NMNAT-1 expression in Hep G2 nuclear extract.

Anti-Nmnat1 antibody [1F7] - ab118270 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F7
All lanes : Anti-Nmnat1 antibody [1F7] (ab118270) at 1/2000 dilutionLane 1 : Non transfected cell lysateLane 2 : Nmnat1 transfected HEK293T cell lysateLysates/proteins at 5 µg per lane.

Anti-Nmnat1 antibody [2642C5a] - ab84855 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2642C5a
Anti-Nmnat1 antibody [2642C5a] (ab84855) + Immunogen (Recombinant Human Nmnat1 fragment)

Anti-Nmnat1 antibody [AT4A2] - ab105017 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone AT4A2
Anti-Nmnat1 antibody [AT4A2] (ab105017) at 1/1000 dilution + HepG2 cell lysate at 35 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique

Anti-Nmnat1 antibody - ab45652 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Nmnat1 antibody (ab45652) at 1 µg/ml + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Nmnat1 antibody - ab45548 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nmnat1 antibody (ab45548) at 1/250 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-NMNAT1 / NMNAT Antibody (clone 1G4) - LS-C337210 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G4
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Anti-NMNAT1 / NMNAT Antibody (aa229-279) IHC-plus™ - LS-B12097 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-NMNAT1 / NMNAT Antibody - LS-C113061 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-NMNAT1 / NMNAT Antibody - LS-C126041 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-NMNAT1 / NMNAT Antibody (aa4-279) - LS-C131073 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-NMNAT1 / NMNAT Antibody (clone 2642C5a) - LS-C184732 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2642C5a

Anti-NMNAT1 / NMNAT Antibody (aa1-279, clone TS106) - LS-C191474 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone TS106

Anti-NMNAT1 / NMNAT Antibody (aa50-99) - LS-C81314 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.

Anti-NMNAT1 / NMNAT Antibody (clone 2642C5a) - LS-C342762 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2642C5a
Left : Western blot analysis of immunized recombinant protein, using anti-NMNAT1 monoclonal antibody. Right : CBB staining of immunized recombinant protein.

Anti-NMNAT1 / NMNAT Antibody (clone 1F7) - LS-C337209 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F7
Anti-NMNAT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NMNAT1.

NMNAT-1 Antibody (1G4) - NBP2-03452 from Novus Biologicals

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G4
Western Blot: Nmnat1 Antibody (1G4) [NBP2-03452] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nmnat1 monoclonal antibody.

NMNAT-1 Antibody - NBP2-32464 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NMNAT-1 Antibody (675112) - MAB5865 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 675112
NMNAT‑1 was detected in perfusion fixed frozen sections of rat brain (cortex) using Mouse Anti-Human NMNAT‑1 Monoclonal Antibody (Catalog # MAB5865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and microglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

NMNAT-1 Antibody - H00064802-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: NMNAT-1 Antibody [H00064802-B01P] - Analysis of NMNAT1 expression in human placenta.

NMNAT-1 Antibody - NBP1-52973 from Novus Biologicals

Prices $299.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NMNAT-1 Antibody [NBP1-52973] - Sample Tissue: Human HCT116 Antibody Dilution: 1.0 ug/ml

NMNAT-1 Antibody - AF5865 from Novus Biologicals

Prices $99.00, $329.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of human muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human NMNAT‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5865) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NMNAT‑1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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