Anti-NMNAT2 antibody
| Name | Anti-NMNAT2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56980 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | ICC/IF ICC/IF WB IHC-P FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: CIPGLWNEAD MEVIVGDFGI VVVPRDAADT DRIMNHSSIL RKYKNNIMVV KDDINHPMSV VSSTKSRLAL QHGDGHVVDY LSQPVIDYIL KSQLYINASG , corresponding to amino acids 208-308 of Human NMNAT2 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | NMNAT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot against tagged recombinant protein immunogen using ab56980 NMNAT2 antibody at 1ug/ml.
ICC/IF image of ab56980 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56980, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab56980 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56980, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing SH-SY5Y cells stained with ab56980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/950_NMNAT2-Primary-antibodies-ab56980-3.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab56980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Anti-NMNAT2 antibody (ab56980) at 2 µg/ml + mouse brain from newborn pup at 40 µgSecondaryGoat Anti-Mouse IgG (H+L)-HRP Conjugate at 1/3000 dilutionJon Gilley, The Babraham Institute, United KingdomSee Abreview
Product References
CREB-activity and nmnat2 transcription are down-regulated prior to - CREB-activity and nmnat2 transcription are down-regulated prior to
Ljungberg MC, Ali YO, Zhu J, Wu CS, Oka K, Zhai RG, Lu HC. Hum Mol Genet. 2012 Jan 15;21(2):251-67.
Endogenous Nmnat2 is an essential survival factor for maintenance of healthy - Endogenous Nmnat2 is an essential survival factor for maintenance of healthy
Gilley J, Coleman MP. PLoS Biol. 2010 Jan 26;8(1):e1000300.