Anti-nmt55 / p54nrb antibody
| Name | Anti-nmt55 / p54nrb antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70335 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Horse, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Orangutan, Elephant |
| Antigen | Synthetic peptide corresponding to a region between residues 350 and 400 of human nmt55/ p54nrb (NP_031389 |
| Description | Rabbit Polyclonal |
| Gene | NONO |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling nmt55 / p54nrb with ab70335 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-nmt55 / p54nrb antibody (ab70335) at 0.04 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µg
Detection of Human nmt55 / p54nrb by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70335 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
ICC/IF image of ab70335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab70335 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70335, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-nmt55 / p54nrb antibody (ab70335) at 0.1 µg/mlLane 1 : NIH3T3 whole cell lysateLane 2 : TCMK-1 whole cell lysateLane 3 : 4T1 whole cell lysateLane 4 : CT26.WT whole cell lysateLane 5 : C6 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique
Product References
Non-catalytic participation of the Pin1 peptidyl-prolyl isomerase domain in - Non-catalytic participation of the Pin1 peptidyl-prolyl isomerase domain in
Innes BT, Bailey ML, Brandl CJ, Shilton BH, Litchfield DW. Front Physiol. 2013 Feb 13;4:18.
Rasd1 modulates the coactivator function of NonO in the cyclic AMP pathway. - Rasd1 modulates the coactivator function of NonO in the cyclic AMP pathway.
Ong SA, Tan JJ, Tew WL, Chen KS. PLoS One. 2011;6(9):e24401.
TNF-alpha suppresses prolyl-4-hydroxylase alpha1 expression via the ASK1-JNK-NonO - TNF-alpha suppresses prolyl-4-hydroxylase alpha1 expression via the ASK1-JNK-NonO
Zhang C, Zhang MX, Shen YH, Burks JK, Zhang Y, Wang J, LeMaire SA, Yoshimura K, Aoki H, Coselli JS, Wang XL. Arterioscler Thromb Vasc Biol. 2007 Aug;27(8):1760-7. Epub 2007 May 3.
A coactivator trap identifies NONO (p54nrb) as a component of the cAMP-signaling - A coactivator trap identifies NONO (p54nrb) as a component of the cAMP-signaling
Amelio AL, Miraglia LJ, Conkright JJ, Mercer BA, Batalov S, Cavett V, Orth AP, Busby J, Hogenesch JB, Conkright MD. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20314-9. Epub 2007 Dec 11.