NONO

Gene Symbol NONO
Entrez Gene 4841
Alt Symbol NMT55, NRB54, P54, P54NRB, PPP1R114
Species Human
Gene Type protein-coding
Description non-POU domain containing, octamer-binding
Other Description 54 kDa nuclear RNA- and DNA-binding protein|55 kDa nuclear protein|DNA-binding p52/p100 complex, 52 kDa subunit|non-POU domain-containing octamer (ATGCAAAT) binding protein|non-POU domain-containing octamer-binding protein|p54(nrb)|protein phosphatase 1, regulatory subunit 114
Swissprots O00201 B7Z4C2 Q15233 Q12786 P30807 Q9BQC5 F5GYZ3 D3DVV4
Accessions CAA72157 EAX05298 EAX05299 EAX05300 Q15233 AK055406 AK291140 BAF83829 AK297144 BAH12508 AU119361 BC002364 AAH02364 BC003129 AAH03129 BC004113 BC010049 BC012141 AAH12141 BC025280 BC025352 BC028299 AAH28299 BC069616 AAH69616 BC069639 AAH69639 CR456761 CAG33042 DA116840 DA779425 L14599 AAC37578 L32558 AAA36727 U02493 AAA03427 U89867 AAC51852 NM_001145408 NP_001138880 NM_001145409 NP_001138881 NM_001145410 NP_001138882 NM_007363 NP_031389
Function DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRC
Subcellular Location Nucleus. Nucleus, nucleolus. Nucleus speckle. Note=Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. {ECO:0000269|PubMed:9341872}.

p54/nrb (C-20) - sc-46221 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p54/nrb (C-20): sc-46221. Western blot analysis of p54/nrb expression in MCF7 (A), A-673 (B), PC-3 (C) and MOLT-4 (D) nuclear extracts.

p54/nrb (E-3) - sc-376806 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p54/nrb (E-3): sc-376806. Western blot analysis of p54/nrb expression in HeLa (A) and MOLT-4 (B) nuclear extracts.

p54/nrb (C-17) - sc-23249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p54/nrb (C-17): sc-23249. Western blot analysis of p54/nrb expression in HeLa (A), MCF7 (B), A673 (C) and PC-3 (D) nuclear extracts.

p54/nrb (F-5) - sc-376804 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p54/nrb (F-5): sc-376804. Western blot analysis of p54/nrb expression in HeLa (A) and MOLT-4 (B) nuclear extracts.

p54/nrb (G-1) - sc-376865 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p54/nrb (G-1): sc-376865. Western blot analysis of p54/nrb expression in HeLa (A) and MOLT-4 (B) nuclear extracts.

p54/nrb (H-85) - sc-67016 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p54/nrb (H-85): sc-67016. Western blot analysis of p54/nrb expression in HeLa (A), MOLT-4 (B) and MCF7 (C) nuclear extracts.

p54/nrb (N-12) - sc-23247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p54/nrb (N-12): sc-23247. Western blot analysis of p54/nrb expression in HeLa (A) and MOLT-4 (B) nuclear extracts.

p54/nrb (B-3) - sc-377421 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p54/nrb (B-3): sc-377421. Western blot analysis of p54/nrb expression in MCF7 (A) and HeLa (B) nuclear extracts.

p54/nrb (A-9) - sc-166704 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p54/nrb (A-9): sc-166704. Western blot analysis of p54/nrb expression in non-transfected: sc-117752 (A) and human p54/nrb transfected: sc-114638 (B) 293T whole cell lysates.

p54/nrb (A-11) - sc-166702 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p54/nrb (A-11): sc-166702. Western blot analysis of p54/nrb expression in HeLa nuclear extract.

p54/nrb (C-16) - sc-46220 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p54/nrb (C-16): sc-46220. Western blot analysis of p54/nrb expression in MCF7 (A), A-673 (B), PC-3 (C) and MOLT-4 (D) nuclear extracts.

p54/nrb (N-13) - sc-46223 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p54/nrb (N-13): sc-46223. Western blot analysis of p54/nrb expression in MCF7 (A), PC-3 (B) and MOLT-4 (C) nuclear extracts.

p54/nrb (3) - sc-136296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p54/nrb (3): sc-136296. Western blot analysis of p54/nrb expression in Jurkat whole cell lysate.

Anti-nmt55 / p54nrb antibody - ab70335 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling nmt55 / p54nrb with ab70335 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.

Anti-nmt55 / p54nrb antibody - ab45359 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-nmt55 / p54nrb antibody (ab45359) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-nmt55 / p54nrb antibody - ab50411 from Abcam

Prices $382.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-nmt55 / p54nrb antibody (ab50411) at 0.1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).

Anti-nmt55 / p54nrb antibody - ab50952 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-nmt55 / p54nrb antibody (ab50952) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

Anti-nmt55 / p54nrb antibody - ab110189 from Abcam

Prices $384.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-nmt55 / p54nrb antibody (ab110189) at 0.1 µg/ml + Mouse brain lysate at 35 µgdeveloped using the ECL technique

Anti-nmt55 / p54nrb antibody - ab195622 from Abcam

Prices $378.00
Sizes 250 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-nmt55 / p54nrb antibody (ab195622) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysate

Anti-nmt55 / p54nrb antibody - ab50938 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-nmt55 / p54nrb antibody (ab50938) at 1.25 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-nmt55 / p54nrb antibody [3F8] - ab150341 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F8
All lanes : Anti-nmt55 / p54nrb antibody [3F8] (ab150341) at 1/200 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY nmt55 / p54nrb Lysates/proteins at 5 µg per lane.

Anti-nmt55 / p54nrb antibody [5F1] - ab131654 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5F1
All lanes : Anti-nmt55 / p54nrb antibody [5F1] (ab131654) at 1/1000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY nmt55 / p54nrb cDNA Lysates/proteins at 5 µg per lane.

Anti-nmt55 / p54nrb antibody [EPR5270] - ab133574 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5270
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labelling nmt55 / p54nrb using ab133574 at a 1/250 dilution.

Anti-NONO / P54NRB Antibody - LS-C333957 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human thyroid cancer using NONO antibody at dilution of 1:200 (200x lens).
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