Anti-nNOS (neuronal) (phospho S1417) antibody

• Supplier: Abcam
• Catalog: ab5583
• Prices: $384.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F WB IHC-F ICC/IF IP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Rabbit, Human, Xenopus, Zebrafish
• Antigen: Synthetic peptide corresponding to Human nNOS (neuronal) aa 1411-1425 (phospho S1417)
• Description: Rabbit Polyclonal
• Gene: NOS1
• Conjugate: Unconjugated

Product images

Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

ab5583 staining nNOS (phospho S1417) in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formalin, permeabilized with 0.25% Triton X-100 and blocked with 3% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/500 in PBS + Triton X-10 + normal donkey serum) for 48 hours at 4°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.See Abreview

Product References

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