NOS1

Gene Symbol NOS1
Entrez Gene 4842
Alt Symbol IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS
Species Human
Gene Type protein-coding
Description nitric oxide synthase 1 (neuronal)
Other Description NOS type I|constitutive NOS|neuronal NOS|nitric oxide synthase, brain|peptidyl-cysteine S-nitrosylase NOS1
Swissprots O75713 E9PH30 P29475
Accessions AAB60654 AAR07069 CCQ42990 EAW98108 EAW98109 EAW98110 P29475 AF446131 AF446132 AF446133 AF446134 AF446135 AF446136 AF446137 AF446138 AF446139 AF446140 AJ004918 CAA06218 AK294435 BAG57679 AK307481 BC010126 BC033208 AAH33208 BC156399 AAI56400 BC172474 AAI72474 BE207961 D16408 BAA03895 EU753241 ACF04132 L02881 AAA36376 U17327 AAA62405 U31466 AAB49040 XM_011538398 XP_011536700 NM_000620 NP_000611 NM_001204213 NP_001191142 NM_001204214 NP_001191143 NM_001204218 NP_001191147
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Subcellular Location Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine {ECO:0000250}. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity). {ECO:0000250}.
Tissue Specificity Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Top Pathways Circadian entrainment, Phagosome, Salivary secretion, Calcium signaling pathway, Amyotrophic lateral sclerosis (ALS)

nNOS (C12H1) Rabbit mAb - 4236 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C12H1
Western blot analysis of extracts from mouse and rat brain using nNOS (C12H1) Rabbit mAb.

nNOS Antibody - 4234 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from mouse and rat brain, using nNOS Antibody.

nNOS (C7D7) Rabbit mAb - 4231 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C7D7
Western blot analysis of extracts from mouse and rat brain using nNOS (C7D7) Rabbit mAb.

NOS (S-16) - sc-49058 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NOS (S-16): sc-49058. Western blot analysis of NOS expression in non-transfected: sc-117752 (A) and human NOS1 transfected: sc-372005 (B) 293T whole cell lysates.

NOS1 (A-11) - sc-5302 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NOS1 (A-11): sc-5302. Western blot analysis of NOS1 expression in mouse brain tissue extract (A) and A-673 whole cell lysate (B).

NOS1 (D-10) - sc-17825 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NOS1 (D-10): sc-17825. Western blot analysis of NOS1 expression in A-673 whole cell lysate.

NOS1 (G-4) - sc-398843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NOS1 (G-4): sc-398843. Western blot analysis of NOS1 expression in mouse brain (A) and human skeletal muscle (B) tissue extracts.

NOS1 (H-299) - sc-8309 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NOS1 (H-299): sc-8309. Western blot analysis of NOS1 expression in rat (A) and mouse (B) brain tissue extracts.

NOS1 (H-7) - sc-55521 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NOS1 (H-7): sc-55521. Western blot analysis of NOS1 expression in A-673 whole cell lysate.

NOS1 (K-20) - sc-1025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NOS1 (K-20): sc-1025. Western blot analysis of NOS1 expression in mouse brain tissue extract.

NOS1 (R-20) - sc-648 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NOS1 (R-20): sc-648. Western blot analysis of NOS1 expression in mouse brain tissue extract.

p-NOS1 (Ser 852) - sc-19826 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-NOS1 (Ser 852): sc-19826. Western blot analysis of p-NOS1 expression in rat testis tissue extract.

Anti-nNOS (neuronal) (phospho S1417) antibody - ab5583 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-nNOS (neuronal) (phospho S1417) antibody - ab90443 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-nNOS (neuronal) (phospho S1417) antibody (ab90443) at 1/500 dilutionLane 1 : SK-N-SH cell lysateLane 2 : SK-N-SH plus Nocodazole cell lysatedeveloped using the ECL technique

Anti-nNOS (neuronal) (phospho S847) antibody - ab16650 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/mlLane 1 : Forebrain (Mouse) Tissue LysateLane 2 : Mouse Cortex Tissue LysateLane 3 : Spinal Cord (Mouse) Tissue LysateLane 4 : Forebrain (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (phospho S847) peptide (ab16981) at 1 µg/mlLane 5 : Mouse Cortex Tissue Lysate with Mouse nNOS (neuronal) (phospho S847) peptide (ab16981) at 1 µg/mlLane 6 : Spinal Cord (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (phospho S847) peptide (ab16981) at 1 µg/mlLane 7 : Forebrain (Mouse) Tissue Lysate with nNOS (neuronal) peptide at 1 µg/mlLane 8 : Mouse Cortex Tissue Lysate with nNOS (neuronal) peptide at 1 µg/mlLane 9 : Spinal Cord (Mouse) Tissue Lysate with nNOS (neuronal) peptide at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)Performed under reducing conditions.

Anti-nNOS (neuronal) (phospho S852) antibody - ab195998 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-nNOS (neuronal) (phospho S852) antibody (ab195998) at 1/500 dilutionLane 1 : A549 whole cell lysates with antigen-specific peptideLane 2 : A549 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-nNOS (neuronal) (phospho S852) antibody - ab194524 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-nNOS (neuronal) (phospho S852) antibody (ab194524) at 1/500 dilutionLane 1 : A549 cell lysate with no antigen specific peptideLane 2 : A549 cell lysate with the antigen specific peptide

Anti-nNOS (neuronal) (phospho S852) antibody - ab58533 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab58533, at 1/50 dilution, staining nNOS (neuronal) in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

Anti-nNOS (neuronal) antibody - ab5586 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (green) showing staining in the cytoplasm and membrane of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5586 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-nNOS (neuronal) antibody - ab3511 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry was performed on normal biopsies of deparaffinized Rat cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing nNOS (ab3511 ) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-nNOS (neuronal) antibody - ab15203 from Abcam

Prices $382.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab15203 staining nNOS (neuronal) in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Sections were deparaffinized, rehydrated and submitted to antigen retrieval and blocked with 5% non-fat dry milk and 0.1% Triton X-100. Samples were incubated with primary antibody (1/200 in PBS containing 2% non-fat dry milk and 0.05% Trition X-00) overnight at 4°C. A Biotin-conjugated anti-rabbit IgG (1/200 in PBS containing 1% NGS for 1 hour at room temperature) was used as the secondary antibody. Left - wild type, Right - Cre+/RTN-1C+. Magnification: 20x.

Anti-nNOS (neuronal) antibody - ab63602 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab63602, at a 1/500 dilution, staining Human nNos (neuronal) in HeLa cells, by Immunofluorescence, with (+) or without (-) immunising peptide.

Anti-nNOS (neuronal) antibody - ab106417 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of nNOS staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106417, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-nNOS (neuronal) antibody - ab79051 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of nNOS (neuronal) in formalin-fixed, paraffin-embedded human skeletal tissue using ab79051 at 5µg/ml.

Anti-nNOS (neuronal) antibody - ab95436 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab95436 at 2µg/ml staining NOS in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). A heat mediated antigen retrieval step was performed.
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