Anti-non-muscle Myosin IIA antibody
| Name | Anti-non-muscle Myosin IIA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55456 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | IP WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: RLKQLKRQLE EAEEEAQRAN ASRRKLQREL EDATETADAM NREVSSLKNK LRRGDLPFVV PRRMARKGAG DGSDEEVDGK ADGAEAKPAE , corresponding to amino acids 131-221 of Human non-muscle Myosin IIA Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | MYH9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot against tagged recombinant protein immunogen using ab55456 non-muscle Myosin IIA antibody at 1ug/ml.
ICC/IF image of ab55456 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55456, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HEK293 cells stained with ab55456 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55456, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/1352_non-muscle-Myosin-IIA-Primary-antibodies-ab55456-3.jpg)
Overlay histogram showing HEK293 cells stained with ab55456 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55456, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Immunofluorescence analysis of plakoglobin-knocked down MCF7 cells, staining non-muscle Myosin IIA with ab55456. Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked in 5% goat serum. Samples were incubated with primary antibody overnight at 4°C. An AlexaFluor®-conjugated anti-mouse IgG was used as the secondary antibody.
Anti-non-muscle Myosin IIA antibody (ab55456) + HeLa
Product References
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Phosphorylation of the myosin IIA tailpiece regulates single myosin IIA molecule - Phosphorylation of the myosin IIA tailpiece regulates single myosin IIA molecule
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Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and - Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and
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Myosin II isoform switching mediates invasiveness after TGF-beta-induced - Myosin II isoform switching mediates invasiveness after TGF-beta-induced
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Exchange protein directly activated by cyclic AMP increases melanoma cell - Exchange protein directly activated by cyclic AMP increases melanoma cell
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Myosin IIA associates with NK cell lytic granules to enable their interaction - Myosin IIA associates with NK cell lytic granules to enable their interaction
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