Anti-NOX1 antibody

• Supplier: Abcam
• Catalog: ab55831
• Prices: $392.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB ELISA
• Species Reactivities: Mouse, Human, Pig
• Antigen: Synthetic peptide corresponding to C terminal residues of human NOX1
• Blocking Peptide: NOX1 peptide
• Description: Rabbit Polyclonal
• Gene: NOX1
• Conjugate: Unconjugated

Product images

ICC/IF image of ab55831 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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