
All lanes : Anti-NOX1 antibody (ab101027) at 1/250 dilutionLane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLane 2 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)Lane 3 : LNCaP human prostate carcinoma cell line lysate Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.