All lanes : Anti-Npas4 antibody (ab93822) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 4 : Mouse Olfactory Bulb LysateLane 5 : Forebrain (Mouse) Tissue LysateLane 6 : Hippocampus (Mouse) Tissue Lysate Lane 7 : Cerebellum Mouse Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 74 kDa (why is the actual band size different from the predicted?)Additional bands at : 100 kDa,43 kDa. We are unsure as to the identity of these extra bands.Exposure time : 150 seconds