NPAS4

Gene Symbol	NPAS4
Entrez Gene	266743
Alt Symbol	Le-PAS, NXF, PASD10, bHLHe79
Species	Human
Gene Type	protein-coding
Description	neuronal PAS domain protein 4
Other Description	HLH-PAS transcription factor NXF\|PAS domain-containing protein 10\|class E basic helix-loop-helix protein 79\|neuronal PAS domain-containing protein 4\|neuronal PAS4
Swissprots	Q8N8S5 B7ZL81 Q8N9Q9 Q8IUM7
Accessions	EAW74525 Q8IUM7 AB049469 BAC19830 AK094025 BAC04271 AK096253 BAC04738 BC105001 AAI05002 BC105003 AAI05004 BC143630 AAI43631 EU446888 ABZ92417 XM_011544938 XP_011543240 XR_949877 NM_178864 NP_849195
Function	Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene. {ECO:0000269\|PubMed:14701734}.
Subcellular Location	Nucleus.
Tissue Specificity	Brain. {ECO:0000269\|PubMed:14701734}.

NPAS4 (L-15) - sc-168789 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NPAS4 (N-12) - sc-168790 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-Npas4 antibody - ab109984 from Abcam

Prices	$376.00
Sizes	200 µl
Host	Goat
Clonality	Polyclonal

All lanes : Anti-Npas4 antibody (ab109984) at 1 µg/mlLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLysates/proteins at 35 µg per lane.

Anti-Npas4 antibody - ab93822 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Npas4 antibody (ab93822) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 4 : Mouse Olfactory Bulb LysateLane 5 : Forebrain (Mouse) Tissue LysateLane 6 : Hippocampus (Mouse) Tissue Lysate Lane 7 : Cerebellum Mouse Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 74 kDa (why is the actual band size different from the predicted?)Additional bands at : 100 kDa,43 kDa. We are unsure as to the identity of these extra bands.Exposure time : 150 seconds

Anti-Npas4 antibody - N-terminal - ab187302 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Npas4 antibody - N-terminal (ab187302) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : mouse brain lysate

Anti-NXF / NPAS4 Antibody (aa603-652) - LS-C120156 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NXF / NPAS4 Antibody (C-Terminus) - LS-C144924 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NXF / NPAS4 Antibody (aa539-588) - LS-C109685 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

NPAS4 antibody LS-C109685 Western blot of Fetal Lung lysate.

Anti-NXF / NPAS4 Antibody (aa149-161) - LS-C139716 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

NPAS4 antibody (0.3 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Npas4 Antibody - 44430002 from Novus Biologicals

Prices	$299.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Npas4 Antibody - NBP1-68863 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Npas4 Antibody (4E4) - H00266743-M09 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4E4

ELISA: Npas4 Antibody (4E4) [H00266743-M09] - Detection limit for recombinant GST tagged NPAS4 is 0.3 ng/ml as a capture antibody.

Anti-NPAS4, C-Terminal antibody produced in rabbit - SAB4501155 from Sigma-Aldrich

Prices	$335.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-NPAS4, C-Terminal: <B>Cat. No. SAB4501155</B>: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-NPAS4, C-Terminal antibody. Cells on the left were treated with the Anti-NPAS4, C-Terminal antibody. Cells on the right (negative control) were treated with both Anti-NPAS4, C-Terminal antibody and the synthesized immunogen peptide.

Anti-NPAS4 antibody produced in goat - SAB2501493 from Sigma-Aldrich

Prices	$376.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-NPAS4 <B>Cat. No. SAB2501493</B>: (0.3 μg/mL) staining of Human Frontal Cortex lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Monoclonal Anti-NPAS4 antibody produced in mouse - SAB1402073 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4E4

<B>Immunoblotting</B><BR/>Monoclonal Anti-NPAS4, antibody produced in mouse, clone 4E4, purified immunoglobulin: <B>Cat. No. SAB1402073</B>: Antibody concentration: 1.0 μg/mL. Specific band of ~37 kDa using immunogen protein lysate.

Anti-NPAS4 antibody produced in rabbit - SAB2101623 from Sigma-Aldrich

Prices	$347.50
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-NPAS4: <B>Cat. No.SAB2101623</B>: NPAS4 in Fetal Lung tissue lysate with NPAS4 antibody at 1.0 μg/mL.

NPAS4 antibody - middle region (ARP32556_P050) - ARP32556_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

NPAS4-Antibody-internal-region - AF3412a from Abgent, a WuXi AppTec company

Prices	$335.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AF3412a (0.3 µg/ml) staining of Human Frontal Cortex lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NPAS4-antibody-middle-region - AI10066 from Abgent, a WuXi AppTec company

Prices	$293.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

NPAS4 antibody - middle region (AI10066) in Fetal Lung cells using Western BlotHost: RabbitTarget Name:NPAS4Sample Tissue:Fetal Lung Lane A:Primary Antibody Lane B:Primary Antibody + Blocking Peptide Primary Antibody Concentration:1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/LaneGel Concentration: 0.12

NPAS4 antibody - GTX87940 from GeneTex

Prices	$319.00
Sizes	100 μg
Host	Rabbit
Clonality	Polyclonal

Anti-NPAS4 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX87940

NPAS4 Antibody (OAAF03924) - OAAF03924 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

NPAS4 Antibody (OAEB01042) - OAEB01042 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

NPAS4 antibody - AP23231PU-N from Acris Antibodies

Prices	$310.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AP23231PU-N NPAS4 antibody staining of Mouse (A) and Rat (B) Brain lysates at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NPAS4 antibody - H00266743-B01P from Acris Antibodies

Prices	$1,530.00
Sizes	500 µg
Host	Mouse
Clonality	Polyclonal

NPAS4 antibody - H00266743-M09 from Acris Antibodies

Prices	$360.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4E4

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