All lanes : Anti-NQO1 antibody (ab97385) at 1/1000 dilutionLane 1 : MOLT4 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97385 at 1/200 dilution. Lower image merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab97385 at 1/500 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian endometriosis tissue, staining NQO1 with ab97385.Antigen retrieval was performed in sodium citrate buffer in a microwave oven. Samples were blocked with rabbit serum for one hour before incubating overnight with primary antibody (1/200). Stainined was detected using DAB.