NQO1

Gene Symbol NQO1
Entrez Gene 1728
Alt Symbol DHQU, DIA4, DTD, NMOR1, NMORI, QR1
Species Human
Gene Type protein-coding
Description NAD(P)H dehydrogenase, quinone 1
Other Description DT-diaphorase|NAD(P)H dehydrogenase [quinone] 1|NAD(P)H:Quinone acceptor oxidoreductase type 1|NAD(P)H:menadione oxidoreductase 1|NAD(P)H:quinone oxidoreductase 1|NAD(P)H:quinone oxireductase|azoreductase|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|diaphorase-4|dioxin-inducible 1|menadione reductase|phylloquinone reductase|quinone reductase 1
Swissprots Q86UK1 P15559 B7ZAD1 B4DNM7 B2R5Y9
Accessions AAB60701 AAP20940 CBX47453 CBX47454 CBX47455 CBX51347 EAW83282 EAW83283 EAW83284 P15559 AK223050 BAD96770 AK297125 BAG59630 AK297979 BAG60289 AK298896 BAG61007 AK307533 AK309513 AK312368 BAG35286 AK316246 BAH14617 AV729122 BC000906 BC007659 AAH07659 BC107739 AAI07740 BE903613 BM052991 BM452267 BM787983 BM828301 BQ775231 CD014005 DC331440 DQ892892 ABM83818 DQ896139 ABM87138 J03934 AAA59940 NM_000903 NP_000894 NM_001025433 NP_001020604 NM_001025434 NP_001020605 NM_001286137 NP_001273066
Function The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Subcellular Location Cytoplasm.
Top Pathways Ubiquinone and other terpenoid-quinone biosynthesis

NQO1 (A180) Mouse mAb - 3187 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone A180
Western blot analysis of extracts from several cell lines using NQO1 (A180) Mouse mAb.

NQO1 (A-5) - sc-271116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NQO1 (A-5): sc-271116. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

NQO1 (F-8) - sc-393736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NQO1 (F-8): sc-393736. Western blot analysis of NQO1 expression in Hep G2 (A), HeLa (B), SW480 (C) and HCT-116 (D) whole cell lysates and human kidney tissue extract (E).

NQO1 (C-19) - sc-16464 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NQO1 (C-19): sc-16464. Western blot analysis of NQO1 expression in Hep G2 whole cell lysate.

NQO1 (H-9) - sc-376023 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NQO1 (H-9): sc-376023. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

NQO1 (R-20) - sc-16463 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NQO1 (R-20): sc-16463. Western blot analysis of NQO1 expression in Hep G2 whole cell lysate.

NQO1 (A180) - sc-32793 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NQO1 (A180): sc-32793. Western blot analysis of NQO1 expression in Hep G2 (A), SW480 (B) and HCT 116 (C) whole cell lysates.

NQO1 (H-90) - sc-25591 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NQO1 (H-90): sc-25591. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

Anti-NQO1 antibody - ab34173 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NQO1 antibody (ab34173) at 1 µg/ml + Human Kidney Whole Tissue Lysate at 20 µgSecondaryIRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-NQO1 antibody - ab2346 from Abcam

Prices $391.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
This image was kindly supplied as part of the review submitted by Lauren Aleksunes. Various mouse liver cytosol samples and human recombinant protein were run on the gel and then incubated with ab2346.

Anti-NQO1 antibody - ab97385 from Abcam

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NQO1 antibody (ab97385) at 1/1000 dilutionLane 1 : MOLT4 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-NQO1 antibody - ab183115 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Human liver cancer tissue labeling NQO1 with ab183115 at 1/25 dilution. (Left image). Right image treated with Immunogen.

Anti-NQO1 antibody - ab166778 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling NQO1 with ab166778 at 3.75 µg/ml.

Anti-NQO1 antibody - ab85253 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NQO1 antibody (ab85253) at 1/50 dilution + NCI-H460 cell line lysate at 35 µg

Anti-NQO1 antibody - ab75342 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NQO1 antibody - ab114064 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-NQO1 antibody (ab114064) at 0.3 µg/ml + Human Kidney lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-NQO1 antibody [4D12] - ab119902 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D12
Anti-NQO1 antibody [4D12] (ab119902) at 1/500 dilution + recombinant protein

Anti-NQO1 antibody [A180] - ab28947 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone A180
IHC image of NQO1 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28947, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NQO1 antibody [A180] (Alexa Fluor® 488) - ab195873 from Abcam

Prices $431.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone A180
Overlay histogram showing HepG2 cells stained with ab195873 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195873, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HepG2 fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NQO1 antibody [A180] (Alexa Fluor® 647) - ab195874 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone A180
Overlay histogram showing HepG2 cells stained with ab195874 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195874, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.This antibody gave a positive signal in HepG2 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NQO1 antibody [A180] (FITC) - ab150221 from Abcam

Prices $394.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone A180

Anti-NQO1 antibody [A180] (HRP) - ab196629 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone A180
Anti-NQO1 antibody [A180] (HRP) (ab196629) at 1/5000 dilution + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 20 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NQO1 antibody [A180] (HRP) - ab82880 from Abcam

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone A180
Anti-NQO1 antibody [A180] (HRP) (ab82880) at 1/100 dilution + HeLa cell lysate

Anti-NQO1 antibody [A180] - BSA and Azide free - ab177022 from Abcam

Prices $368.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone A180

Anti-NQO1 antibody [EPR3309] - ab80588 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3309
All lanes : Anti-NQO1 antibody [EPR3309] (ab80588) at 1/20000 dilution (unpurified)Lane 1 : SH-SY5Y cell lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
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