![Overlay histogram showing HeLa cells stained with ab115552 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115552, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/1861_ab115552-6-ab115552FC.jpg)
Overlay histogram showing HeLa cells stained with ab115552 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115552, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-NR1D1 antibody [4F6] (ab115552) + HeLa nuclear extract](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/1862_NR1D1-Primary-antibodies-ab115552-1.jpg)
Anti-NR1D1 antibody [4F6] (ab115552) + HeLa nuclear extract
![Anti-NR1D1 antibody [4F6] (ab115552) + NIH3T3 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/1863_NR1D1-Primary-antibodies-ab115552-2.jpg)
Anti-NR1D1 antibody [4F6] (ab115552) + NIH3T3 cell lysate
ab115552, at 5µg/ml, staining NR1D1 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115552 at 10µg/ml dilution staining NR1D1 in HeLa cells by Immunofluorescence.
Detection limit for recombinant tagged NR1D1 is approximately 1 ng/ml as a capture antibody.